基于血凝素裂解位点的DNA条形码在A型流感病毒分型中的应用
Application of DNA Barcoding Based on Hemagglutinin Cleavage Site for Influenza A Virus Sub-typing
  
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中文摘要:
      为了建立一种快速鉴别AIV的HA亚型的RT-PCR方法,本研究探讨了流感病毒血凝素裂解位点基因作为DNA条形码对A型流感病毒进行亚型鉴定的可行性。通过生物信息学方法对16种A型流感病毒的血凝素基因全长进行比对,在HA裂解位点的两端高度保守区设计一对DNA条形码引物,RT-PCR扩增获得约170bp的核苷酸片段。利用MEGA5.0软件构建N-J系统树并计算亚型间及亚型内平均遗传距离。结果显示,不同A型流感病毒裂解位点氨基酸具有单系性,每一亚型可分别形成各自独立的分支。不同亚型间平均遗传距离为0.277,相同亚型内平均遗传距离0.032。研究表明,利用DNA 条形码技术可在A型流感病毒分型方面进行有效的分子分类鉴定。
英文摘要:
      The feasibility of the hemagglutinin cleavage sites used as DNA barcoding to subtype influenza A virus was explored in the study. According to the alignment of the hemagglutinin gene of 16 subtypes of influenza A virus by bioinformatics means,a pair of primers were designed on both terminals of highly conserved of hemagglutinin cleavage site.170bp products were obtained by RT-PCR. Then phylogenetic tree was constructed and the average intra-type variation and inter-type divergence were calculated with MEGA5.0 software. The phylogenetic tree revealed that amino acid composition of cleavage site was of monophyly in each subtype. The average genetic distance of inter-type was 0.277,and intra-type genetic distance was 0.032. It was concluded that the hemagglutinin DNA barcoding can be used for subtyping influenza A virus effectively.
作者单位
孟慧芝,孙涛,岳志芹,王文斌  
中文关键词:  A型流感病毒  血凝素(HA)  DNA条形码  亚型鉴定
英文关键词:influenza A virus  hemagglutinin(HA)  DNA barcoding technology  subtype
基金项目:国家科技支撑计划(2012BAK11B00)
DOI:
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