园养大袋鼠“粗颌病”的病原分离与鉴定
Pathogen Isolation and Identification for“Lumpy Jaw”of Kangaroos in Zoos
  
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中文摘要:
      园养大袋鼠“粗颌病”是一种由齿槽感染引发顽固性牙周炎,导致出现牙龈溃烂并形成溃疡、窦道,严重时颌面部肿胀、坏死,上下颌骨不可逆性变形等一系列临床症状为特征的口炎综合征。本研究对江苏省南京市红山森林动物园发生“粗颌病”的两种园养大袋鼠进行病原分离与鉴定,从发病大袋鼠的病变部位分离出3株细菌。依据形态特征和培养特性观察,以及生化试验和耐药性检测,初步判定其中1株为粪肠球菌(Enterococcus faecalis),另2株为肺炎克雷伯菌(Klebsiella pnenmoniae)。通过全自动药敏分析和小鼠致病性试验,对分离菌株进一步鉴定,结果发现与初判结果一致。小鼠口腔感染试验可以模拟复制出发病袋鼠下颌肿胀的病变特征,只是一般注射剂量不能致死小鼠。
英文摘要:
      “Lumpy Jaw”of kangaroos in zoos is an oral inflammation syndrome caused by alveolus infection,which was characterized by refractory periodontitis,ulcerated gums resulting in ulcer and sinus,swelling and necrosis in maxillofacial region and irreversible malposition of maxillary and mandible seriously. In this study,the pathogens of “Lumpy Jaw” in park kangaroos from Nanjing Hongshan Forest Zoo were isolated and identified. Three strains of bacteria were isolated and were identified as Enterococcus faecalis and Klebsiella pnenmoniae,respectively,by their morphology,cultural characteristics,biochemical characteristics and drug sensitivity. By full-automatic analysis of drug sensitivity and pathogenicity in mice experiment,further identification of the isolated strains were carried out,and the results were consistent with the former. The oral infection of mice experiment showed that the symptoms of “Lumpy Jaw” in the mice could be simulated,but general dozes could not contribute to death of mice.
作者单位
李照伟,刘霞,费荣梅,陈蓉,王素春  
中文关键词:  袋鼠  粗颌病  病原分离与鉴定  粪肠球菌  肺炎克雷伯菌
英文关键词:kangaroo  Lumpy Jaw  pathogen isolation and identification  Enterococcus faecalis  Klebsiella pnenmoniae
基金项目:科技部科技基础性专项(SQ2012FY3260033)
DOI:10.3969/j.issn.1005-944X.2017.09.028
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