一起石斑鱼苗病毒性神经坏死病的诊断
Diagnose on an Outbreak of Viral Nervous Necrosi of Grouper Fry
  
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中文摘要:
      2017年6—7月,福建省东山县某石斑鱼养殖场多批20~60日龄杂交石斑鱼苗发病,临床表现为游泳行为改变,致盲、打转,鱼体发黑,不食,继而死亡,死亡率高达90%以上。多次应用抗生素治疗无效,寄生虫、细菌学检查为阴性。应用世界动物卫生组织(OIE)推荐的检测引物,采用RT-PCR检测方法扩增出神经坏死病毒(NNV)特异性的421 bp基因片段,以此诊断为病毒性神经坏死病(VNN)。
英文摘要:
      During the period from June to July in 2017,an outbreak of disease was reported,with clinical symptoms of abnormal swimming behavior,blind,skin darkening,anorexia,and died in many batches of 20-60-day-old hybrid groupers in a fish farm of Dongshan County,Fujian Province. The mortality was over 90%. Parasites and bacteriological tests were negative and repeated antibiotic treatment was ineffective. The detection primer for pathogen of VNN which was recommended by OIE was carried out,the specific 421 bp gene fragment of NNV was amplified by RT-PCR detection,which indicated that VNV was pathogens causing mass mortality of Grouper fry.
作者单位
陈晖,肖颖,方成俊,张东斌,余晓薇  
中文关键词:  杂交石斑鱼苗  病毒性神经坏死病  寄生虫学检查  细菌学检查  RT-PCR
英文关键词:Hybrid grouper fry  Viral nervous necrosis  incidence  parasites test  bacteriological test  RT-PCR
基金项目:
DOI:10.3969/j.issn.1005-944X.2018.01.028
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