定点屠宰场(点)猪肉中三种主要食源性致病菌污染情况调查
Investigation on Pork Contamination of three Kinds of Main Food-borne Pathogens in Pork of Fixed-pointed Slaughterhouses
  
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中文摘要:
      为了解本地生猪定点屠宰场(点)猪肉中的沙门氏菌、大肠杆菌O157:H7、单增李斯特菌3种食源性致病菌污染情况,对10个场(点)出场的猪肉进行抽样,采用沙门氏菌测试片、大肠杆菌O157:H7测试片及李斯特菌检测板进行快速检测,再用国标法对快速检测出的阳性样品进行复检鉴定。结果显示:50份样本中,检出沙门氏菌阳性7份,阳性检出率为14%;20份样本中,检出大肠杆菌O157:H7阳性1份,阳性检出率为5%,检出单增李斯特菌阳性5份,阳性检出率为25%。此外,还发现样本中存在细菌的混合污染,其中沙门氏菌和单增李斯特菌双阳性2份,3种菌全阳性1份。调查结果表明:本地屠宰场出场猪肉中,这3种主要食源致病菌污染情况较严重,特别是小型屠宰点,应引起监管部门重视,加快推进屠宰企业转型升级;快速测试片的检测结果准确性偏低,特异性差,需要尽快建立一种快速特异的检测方法,用于屠宰场的致病菌快速检测。
英文摘要:
      In order to recognize the contamination situation of three food-borne pathogens of Salmonella,E. coli O157:H7 and Listeria monocytogenes in local fixed-point slaughterhouses,pork samples from 10 slaughterhouses were collected and used for rapid detection. Then the national standard method was used for repeat detection towards the positive samples. Results showed that,7 of 50 samples were detected positive for Salmonella,the positive detection rate was 14%,1 of the 20 samples was detected positive for E. coli O157:H7,the positive detection rate was 5%,5 of the 20 samples were detected positive for Listeria monocytogenes,the positive detection rate was 25%. In addition,it was found that there existed mixed contamination of bacteria,2 samples showed positive results for both Salmonella and Listeria monocytogenes,I sample showed all positive results for 3 kinds of bacteria. The results indicated that the pollution of three kinds of main food-borne pathogens in pork was serious,especially in small slaughtering sites,hence more attention should be paid by supervision authorities,transformation and upgrading of the slaughtering enterprises should be accelerated. Besides,the accuracy of rapid detection strips was low and the specificity was poor,and it was necessary to establish a rapid and specific detection method as soon as possible for rapid detection of pathogenic bacteria in the slaughterhouses.
作者单位
邱燕,温贵兰,张升波  
中文关键词:  屠宰场  猪肉  沙门氏菌  大肠杆菌O157:H7  单增李斯特菌
英文关键词:slaughterhouse  pork  Salmonella  E. coli O157:H7  Listeria monocytogenes
基金项目:
DOI:10.3969/j.issn.1005-944X.2018.08.006
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