江苏省盐城市3株白羽肉鸭源鹅细小病毒的分离鉴定及其VP3基因遗传进化分析
Isolation and Identification of 3 Strains of Parvovirus in White-feathered Broiler Ducks in Yancheng City of Jiangsu Province and Genetic Evolution Analysis on Their VP3 Genes
  
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中文摘要:
      为分离鉴定导致2017年江苏省盐城市樱桃谷鸭出现短喙、长舌、生长抑制以及死淘率迅速增加的病原,研究其基因遗传进化特点,探讨临床解决方案,用PCR方法,对这一地区3家养鸭场的发病鸭进行检测;对鹅细小病毒阳性样品进行病毒分离,并对其VP3基因进行扩增测序和遗传进化分析。结果显示:从发病鸭中分离到3株可引起鸭短喙与矮小综合征的病毒,分别命名为YC1、SQ1、SQ2;3株病毒VP3基因与我国樱桃谷鸭发生的鸭源鹅细小病毒核苷酸同源性在99%以上,与番鸭中分离的短喙与矮小综合征病毒核苷酸同源性为95%~99%,与经典小鹅瘟核苷酸同源性也在95%以上。结果表明,这3家养鸭场暴发的短喙与矮小综合征均由新型鹅细小病毒感染所导致。该毒株与欧洲分离株的亲缘关系更近,提示这3株病毒可能通过引种传入我国,或者由欧洲分离株进化而来。
英文摘要:
      In order to isolate and identify the pathogens that caused the occurrence of short beaks,protruding tongues,growth inhibition and rapid increase of the death and elimination rate in the Cherry Valley ducks,study the genetic evolution characteristics of related genes,so as to explore the clinical solutions,PCR method was used to detect the pathogens in diseased ducks in 3 farms,and virus isolation was carried out towards the samples which were detected parvovirus-positive,then VP3 genes of isolated viruses were sequenced and analyzed. The results showed that 3 strains of virus were isolated,which were respectively named as YC1、SQ1 and SQ2. According to the sequence alignment,the nucleotide homology of VP3 genes between these 3 strains and duck-derived goose parvovirus which was isolated from Cherry Valley ducks of China before was over 99%;when compared with the virus isolated from Muscovy ducks,the homology was 95% to 99%,while it was over 95% when compared with the classical gosling plague virus. Results showed that,the outbreak of short beak and dwarf syndrome(SBDS)in the dark farms was caused by new duck-derived goose parvovirus,the isolates were more closely related to virus isolated from Europe,indicating that the 3 isolated strains could be introduced into China,or evolved from European isolates.
作者单位
李亚非,潘金金,陈洪益,陆红霞,刘东,刘红祥,陈先亮,徐全刚  
中文关键词:  鹅细小病毒  樱桃谷肉鸭  VP3基因  同源性分析  盐城
英文关键词:goose parvovirus  Cherry Valley broiler duck  VP3 gene  homology analysis  Yancheng
基金项目:国家重点研发计划项目(2017YFC1200500)
DOI:10.3969/j.issn.1005-944X.2018.08.022
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