两种奶牛结核病检测方法与PPD检测符合率比较
Comparative Analysis on the Coincidence Rates between PPD and Two Other Methods in Cow Tuberculosis Detection
  
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中文摘要:
      对新疆某规模化奶牛场的2 106头奶牛用PPD进行普检,共检出结核病阳性牛26头;随机采集20头阴性牛和26头阳性牛抗凝血和全血,进行牛结核γ-干扰素ELISA试验和胶体金检测,然后将检测结果与PPD检测结果进行比对。结果显示:3种方法检测均为阳性的16份,均为阴性的10份,总符合率为57%。PPD和γ-干扰素ELISA相比,均为阳性的23份,阳性符合率为88%(23/26),均为阴性的11份,阴性符合率为55%(11/20);PPD和胶体金检测相比,均为阳性的19份,阳性符合率为73%(19/26),均为阴性的19份,阴性符合率为95%(19/20)。结果表明:3种检测方法的符合率较低;每种检测方法各有优缺点,γ-干扰素ELISA敏感性较高,而胶体金特异性较高。因此,在实际操作中应根据需要,合理选择相应检测方法。
英文摘要:
      In this study,using the PPD method,2 106 cows in a large-scale dairy farm in Xinjiang were tested,a total of 26 cows were detected tuberculosis(TB)positive. Then whole blood derived from 20 TB-negative cows and 26 TB-positive cows were randomly collected to carry out γ-interferon ELISA test and colloidal gold test. Then the detection results were compared with PPD method. According to the comparative results,there were 16 samples detected TB-positive and 10 samples detected negative by both three methods,the detection coincidence rate was 57%. For the comparison between PPD and γ-interferon method,23 samples were detected TB-positive,with a positive coincidence rate of 88%(23/26);11 samples were detected TB-negative,with a negative coincidence rate of 55%(11/20). For the comparison between PPD and colloidal gold test,19 samples were detected positive,with a positive coincidence rate of 73%(19/26),19 samples were detected negative,with a negative rate of 95%(19/20). As a conclusion,the coincidence rates among the three methods for TB detection were relatively low. Each method had its advantage and disadvantage,such as the sensitivity of γ-interferon ELISA test was highest,while colloidal gold test possessed the highest specificity. Hence,the detection method should be reasonable selected on the basis of actual requirements and needs.
作者单位
石琴,袁立岗,蒲敬伟,沙丽,柳炜  
中文关键词:  奶牛结核病  PPD  γ-干扰素  胶体金  符合率
英文关键词:cow tuberculosis  PPD  γ-interferon  colloidal gold test  coincidence rate
基金项目:新疆生产建设兵团十二师科技专项(NYJH2013010)
DOI:10.3969/j.issn.1005-944X.2018.10.023
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