为了解我国禽源沙门氏菌的流行和耐药情况及其血清型、ST型和耐药性之间的关系，对2008—2017年，在我国部分地区分离的90株禽源沙门氏菌，用血清凝集方法鉴定其血清型，用微量肉汤稀释法对部分菌株进行药物敏感性试验；经PCR扩增、测序和比对查询，得到90株菌的ST型，并用BioNumerics 7.6分析MLST分型及其亲缘关系；利用统计学方法，对分离株的血清型、ST型和耐药性进行差异显著性和相关性分析。血清型鉴定结果显示：90株禽源沙门氏菌可分为16种血清型，其中S. Enteritidis为优势血清型。71株菌株的药物敏感性试验结果显示：多重耐药菌株占比90.14%（64/71），对氨苄西林（78.87 %）和磺胺异噁唑（77.46 %）的耐药率较高，仅S. Indiana菌株的ST17型对头孢他啶耐药。其他相关分析结果显示：分离菌株对氨苄西林等7种药物的耐药率显著上升（P＜0.05），而对黏菌素耐药率下降极显著（P＜0.01）；鉴定出14种ST型，其中ST11为优势型；最小生成树显示，仅有一个ST complex，菌株来自7个省市；S. Indiana的耐药率显著高于S. Enteritidis（P＜0.05），且其ST17所耐药物种类多于其他ST型。结果表明：我国禽源沙门氏菌的优势血清型是S. Enteritidis，优势ST型为ST11，其血清型和ST型有一定的相关性，且不同地区分离株之间有一定的亲缘关系；分离株对不同药物的耐药性存在差异，且耐药谱和耐药率与血清型相关，S. Indiana菌株与S. Enteritidis相比更易形成耐药菌株。
In order to recognize the prevalence and antimicrobial resistance of poultry salmonella in China and the relationship between its serotype，ST type and antimicrobial resistance，90 strains of poultry salmonella isolates from some regions in China collected from 2008 to 2017 were identified by serum agglutination test，then the drug sensitivity test was carried out towards some strains by broth microdilution method. Through PCR amplification，sequencing and comparison query，ST types of 90 strains were detected，and the MLST subtypes and their genetic relationships were analyzed by BioNumerics 7.6. Then the difference and correlation of the serotype，ST type and antimicrobial resistance of the isolates were analyzed by use of the statistical method. The results showed that 90 strains of poultry salmonella could be divided into 16 serotypes，of which S. Enteritidis was dominant. Based on the drug sensitivity test results of 71 strains，it was found that multiple-resistant strains accounted for 90.14%（64/71），these strains showed high antimicrobial resistance against ampicillin（78.87 %）and sulfisoxazole（77.46 %），and only ST17 subtype of S. Indiana strain was resistant to ceftazidime. Besides，it was indicated that，based on the results of correlation analysis，the resistance rate of the isolates against ampicillin and other drugs increased significantly（P＜0.05），while the resistance to colistin decreased sharply（P＜0.01）. A total of 14 kinds of ST subtypes were identified，and ST11 subtype was dominant. As indicated by the minimum spanning tree，there was only one ST complex，and the strains were collected from 7 provinces/cities；the resistance rate of S. Indiana was obviously higher than that of S. Enteritidis（P＜0.05），and its ST11 subtype was resistant to more drugs than other ST types. The results showed the predominant serotype of poultry salmonella in China was S. Enteritidis，and the predominant ST type was ST11，which indicated there was a certain correlation between the serotype and ST type，with some genetic relationship among the isolates in different regions；the resistance of the isolates against different drugs was also different，and the resistant spectrum and rate were correlated with the serotype，the strain of S. Indiana was more likely to produce resistance compared to the S. Enteritidis.