In April 2019，100 piglets of 5-week old were purchased from a breeding farm by a fattening farm in Xintai City of Shandong Province. Subsequently，some symptoms such as lethargy，vomiting，diarrhea or even death appeared in more than 40 piglets after one week，a total of 28 piglets died，with a mortality rate of 68%，which was finally confirmed as the mixed infection with pseudorabies virus and Haemophilus parasuis through laboratory comprehensive diagnosis including pathological autopsy，histopathological examination and etiological test. Then the gE gene of isolated pseudorabies virus was sequenced and analyzed，and high homology of the isolate with current epidemic strain was found，which provided a basis for selection and efficient application of the vaccine. Based on the above results，corresponding control measures were formulated，and the outbreak was rapidly controlled. Furthermore，the reasons，clinical characters and pathological changes of the cases were discussed and analyzed in the paper，which were of significance for diagnosis and control of similar cases.