In recent years，sheep pasteurella multocida has occasionally appeared in Hulunbuir of Inner Mongolia Autonomous Region. In order to recognize the prevalence status of its capsular serotype，a molecular biology diagnostic method for pasteurella multocida was established，and taking pathological tissues from diseased sheep as research object，an epidemiological investigation was carried out. Meanwhile，the antibiotic susceptibility of isolated strains were detected by K-B agar diffusion method to find any sensitive drugs. The results showed that 12 strains of serogroup B and 9 of serogroup D were isolated from 35 pathological lung tissues，with no any other serogroup；the isolated strains were with the highest antibiotic resistance to penicillin and streptomycin，but were sensitive to chloramphenicol，tetracycline，norfloxacin and ciprofloxacin. As a conclusion，the sheep pasteurella multocida that was prevalent in Hulunbuir mainly included serogroups of B and D，and the isolated strains showed higher antibiotic resistance to penicillin and streptomycin，hence the application of antibiotics should be guided and supervised. In this paper，the mainly prevalent serogroup of pasteurella multocida was identified，and the targeted sensitive drugs were also confirmed，which not only provided effective technical supports for control of the disease，but also laid a foundation for epidemiological surveillance over the disease and relevant study on genetic diversity.