In order to recognize the infection status of wild porcine pseudorabies virus（PRV）in Pu'er City of Yunnan Province，2 035 swine serum samples were collected from 490 free-range households and 71 large-scale farms in 10 counties（districts）from 2017 to 2018，and gE antibodies against PRV were detected by gE-ELISA. The results showed that the average positive rate of gE antibody was 1.92%，the rate was 2.26% for large-scale farms，1.77% for free-range households，and 3.41% for breeding herds. According to the analysis of statistical data，the difference of infection rates between large-scale farms and free-range households was not significant（P＞0.05），while an obvious difference was observed in the infection rates between winter-spring season and other seasons，and between different-age pigs（more than 10 months or not）（P＜0.05）. In conclusion，the wild PRV was prevalent to a lesser extent in Pu'er City，with a small infection range，but it was necessary to intensively implement prevention and control measures，especially for breeding herds and in the seasons（winter and spring）with high incidence. In this study，the prevalence and spreading range of wild PRV，as well as the seasons with high incidence and pig herds at high risk were basically identified，which provided some references for formulating and implementing related purification strategies in the city.