In order to study the proliferation and physicochemical properties of goose-origin Avipoxviru，goose-origin Avipoxvirus identified in China was isolated and cultured by use of chicken embryo and its physicochemical properties were studied. The goose skin pox samples，after being ground，were inoculated into SPF chicken embryos for continous generation culture，it was confirmed that，by pathological examination and PCR assay，the virus could proliferate in chicken embryos，and the lesions and virus titer tended to be stable after 5 consecutive generations. The typical lesions of avian pox，such as，thickening edema of chorioallantoic membrane and white pox marks，could be observed in chicken embryos. The pathological chorioallantoic membrane was observed by electron microscope，and virus inclusion bodies and typical Avipoxvirus particles were found. The physicochemical properties were identified by use of the culture system，it was found that the virus was sensitive to heat（55 ℃），acid（pH3），alkali（pH11），trypsin，ether and chloroform. The chicken embryo culture system for goose-origin Avipoxvirus introduced in this study was firstly established，and physicochemical properties were identified，which provided necessary technical foundation for systematic study on biological characteristics and technology for control of the virus.