In order to compare the efficiency of various nucleic acid extraction kits for Brucella，the nucleic acids of 16 clinical tissue samples and Brucella inactivated bacterial solution were respectively extracted by six kinds of kits（three of immunomagnetic bead kits and column kits respectively），and their efficiencies were evaluated by real-time PCR. Nucleic acids in the solution with dilutability of 102 and 104 were extracted by the kits，it was found that the Ct value of the extracted products of immunomagnetic bead kits was lower than that of column kits，and the variable coefficient of the same batch was less than 5%；the nucleic acids of all tissue samples could be extracted by two kinds of column kits，the nucleic acids of 14 samples could be extracted by two kinds of immunomagnetic bead kits，and nucleic acids of 12 and 3 samples could be extracted respectively by the remaining kits（one kind of bead kit，one kind of column kit）. It was concluded that the nucleic acids in solution could be effectively extracted by all the experimented kits，in which，the extraction efficiency of immunomagnetic bead kits were slightly superior to the column kits，while the efficiency of viral DNA column kits for clinical tissue samples was slightly higher than that of immunomagnetic bead kits，but both of them were not fitted to extract Brucella nucleic acids from clinical tissue samples. Therefore，appropriate kits should be used subject to laboratory conditions，number of samples，urgency of cases and other factors. In this paper，some references were provided to select an appropriate method to extract the nucleic acids of Brucella.