Haemophilus parasuis has brought huge losses to pig industry due to its high morbidity and mortality. In order to confirm the disease timely，the research progress on relevant laboratory testing technologies as well as their advantages and disadvantages were summarized from the aspects of isolation and identification of bacteria，serological detection and molecular biological detection. Specifically，the method of isolation and identification of bacteria was with strong specificity，but was complex and time-consuming for operation，which would require for more instruments and fail to meet the needs of rapid detection；the serological methods including enzyme linked immunosorbent assay（ELISA），indirect hemagglutination assay（IHA）and complement fixation test（CF）were simple and rapid，mainly appropriate for early diagnosis and screening，but their specificity and sensitivity were relatively low. In recent years，with rapid development of molecular biotechnology，a variety of molecular biological methods including PCR，fluorescence quantitative PCR，digital PCR and loop mediated isothermal amplification（LAMP）had been established，which were sensitive，rapid，specific and stable，especially，PCR and fluorescent quantitative PCR had been widely used in practice. But digital PCR and LAMP still needed to be further optimized. In conclusion，an effective technical support was provided for detection of the disease and epidemiological investigation through establishing and improving a variety of laboratory testing methods with the advantages of good specificity and repeatability，high sensitivity and rapid detection speed.