肉种鸡场鸡毒支原体感染的治疗效果评价
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潍坊市科学技术发展计划项目(2017GX051);潍坊市科学技术发展计划项目(2017GX057)


Evaluation on the Treatment Effect against the Infection with Mycoplasma gallisepticum in a Broiler Breeder Farm
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    摘要:

    山东省某一大型父母代肉种鸡场饲养的肉鸡在约44周龄时证实发生鸡毒支原体(MG)感染。为评价有关敏感药物对MG感染的治疗效果,经对该场分离的MG毒株进行最低抑菌浓度(MIC)测定,选择敏感药物恩诺沙星和泰妙菌素实施对比性治疗,采用套式PCR检测治疗1个疗程后肉种鸡所产种蛋和种蛋孵化后期死胚中的MG感染情况,计算感染率。MIC测定结果显示:MIC≤5 μg/mL的药物中,敏感性从高到低依次是沃尼妙林、强力霉素、泰妙菌素、恩诺沙星、大观霉素、泰乐菌素和氟苯尼考。对比性治疗结果显示:恩诺沙星和泰妙菌素两种药物不能完全清除MG对种蛋的感染,但可减少MG的种蛋传播,与未治疗的对照组相比感染率下降极其明显(P=0.006,<0.01);两种药物治疗1个疗程后的未上孵种蛋和种蛋孵化后期死胚的MG感染率差异不显著(P=0.391,>0.05),表明两种药物治疗效果相近。结果表明:恩诺沙星和泰妙菌素两种药物对MG感染治疗有效,而且效果相近;通过套式PCR检测,计算种蛋或孵化后期死胚的MG感染率能直接、快速筛选出阻止或减少MG经蛋传播的敏感药物,评价不同抗菌药物的治疗效果。本研究为种鸡场MG感染和防控效果评价模式的建立提供了参考。

    Abstract:

    It was confirmed that the parental broiler breeders were infected with Mycoplasma gallisepticum(MG)in a large farm when they were at about 44 weeks old. In order to evaluate the treatment effect of relevant sensitive drugs,the minimal inhibitory concentration(MIC)of the MG strain isolated from the farm was determined,Enrofloxacin and Tiamulin were respectively used for comparative treatment,then the infection rate was calculated according to the status of MG infection in hatching eggs or dead embryos at late hatching stage after one course of treatment by nested PCR. It was shown that,by MIC determination,for the drugs with MIC≤5 μg/mL,the sensitivity of Vorimillin,Doxycycline,Temicin,Enrofloxacin,Spectinomycin,Tylosin and Florfenicol was successively decreasing. After comparative treatment,it was shown that MG infection in hatching eggs could not be completely cleaned up by the two drugs,but its spreading could be reduced,and its infection rate decreased significantly(P=0.006,<0.01)compared with the untreated control group;after one course of treatment by the two drugs,the infection rates of unhatched eggs and dead embryos at late hatching stage had no significant difference(P=0.391,>0.05),indicating similar effect of the two drugs. It was concluded that the sensitive drugs that could prevent or reduce the spreading of MG via eggs were rapidly selected through calculating the infection rate of hatching eggs or dead embryos by nested PCR,so as to evaluate the treatment effect of various antibiotics. Some references were hereby provided to establish an evaluation model for MG infection and control effect.

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隋兆峰,李婧,朱俊平.肉种鸡场鸡毒支原体感染的治疗效果评价[J].《中国动物检疫》编辑部,2020,37(9):96-100.

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  • 在线发布日期: 2020-09-02
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