云南省边境地区送检牛血清样品蓝舌病病毒抗体检测与血清型鉴定
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云南省技术创新人才培养对象项目(2018HB076);国家重点研发计划项目(2017YFC1200502)


Detection of Bluetongue Virus Antibodies in Bovine Serum Samples from Border Areas of Yunnan Province and Relevant Serotype Identification
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    摘要:

    为了解云南边境地区的蓝舌病流行和分布情况,采用竞争ELISA方法,2019年对云南省边境地区5个州市12个县(市)的1 580份牛血清样品进行蓝舌病病毒抗体检测,同时从检出的阳性血清中,每县(市)随机选取15~55份,采用微量血清中和试验进行血清型鉴定。结果显示:12个边境县(市)均检出阳性血清样品,但有一定的地区差异,其中芒市最高(94.1%),沧源县最低(17.0%);本地牛和境外牛血清样品阳性率差异不大(P>0.05),均在70.0%以上;各县(市)均存在5个以上血清型的交叉感染。结果表明,蓝舌病在云南边境地区广泛存在,且呈多种血清型混合流行态势,需要持续开展血清学调查。本研究为我国西南边境地区蓝舌病防控提供了数据参考。

    Abstract:

    In order to investigate the prevalence and distribution of bluetongue disease in border areas of Yunnan Province,1 580 cattle serum samples collected from 12 counties(cities)of 5 prefectures(municipalities)in border areas in 2019 were tested for bluetongue virus antibodies. For the positive serum samples,15 to 55 samples were randomly selected from each county(city)to identify serotypes by micro-serum neutralization test. The results showed that positive serum samples were detected out from all the 12 border counties(cities),with certain regional difference,among which,maximum positive rate occurred in Mang City(94.1%)and minimum rate in Cangyuan County(17.0%). The serum positive rate of local cattle was slightly different from that of foreign cattle(P>0.05),both of them were higher than 70.0%. Mixed infection of more than 5 serotypes was present in all counties(cities). It was concluded that bluetongue disease was widely distributed in the border areas,and tended to spread with mixed serotypes,so serological investigation should be continued to carry out. Some data references were provided for the prevention and control of the disease in the border areas in Yunnan Province.

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寇美玲,谢佳芮,李乐,苗海生.云南省边境地区送检牛血清样品蓝舌病病毒抗体检测与血清型鉴定[J].《中国动物检疫》编辑部,2020,37(10):16-20.

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  • 在线发布日期: 2020-09-29
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