In order to investigate the prevalence and distribution of bluetongue disease in border areas of Yunnan Province，1 580 cattle serum samples collected from 12 counties（cities）of 5 prefectures（municipalities）in border areas in 2019 were tested for bluetongue virus antibodies. For the positive serum samples，15 to 55 samples were randomly selected from each county（city）to identify serotypes by micro-serum neutralization test. The results showed that positive serum samples were detected out from all the 12 border counties（cities），with certain regional difference，among which，maximum positive rate occurred in Mang City（94.1%）and minimum rate in Cangyuan County（17.0%）. The serum positive rate of local cattle was slightly different from that of foreign cattle（P＞0.05），both of them were higher than 70.0%. Mixed infection of more than 5 serotypes was present in all counties（cities）. It was concluded that bluetongue disease was widely distributed in the border areas，and tended to spread with mixed serotypes，so serological investigation should be continued to carry out. Some data references were provided for the prevention and control of the disease in the border areas in Yunnan Province.