吉林和黑龙江两省猪鸡源产ESBLs大肠杆菌耐药检测与优势基因型分析
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国家重点研发计划项目(2018YFD0500505)


Drug Resistance Testing for ESBLs-producing E. coli Derived from Chicken and Swine in Jilin and Heilongjiang and Relevant Analysis on Dominant Genotypes
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    摘要:

    为了解吉林和黑龙江两省猪鸡源产超广谱β-内酰胺酶(ESBLs)大肠杆菌菌株的流行分布及耐药特征,分析其优势基因型和基因亚型,依据美国临床实验室标准化委员会(CLSI)标准,对分离自吉林和黑龙江两省的210株大肠杆菌进行ESBLs表型检测,采用微量肉汤稀释法和PCR方法进行药物敏感性试验和耐药基因检测,采用SPSS软件中的卡方检验方法进行数据处理与统计分析。结果显示:①产ESBLs菌株检出率为34.76%,两省检出率差异不显著(P=0.54),鸡源产ESBLs菌株检出率极显著高于猪源(P<0.01)。②210株大肠杆菌对四环素(90.95%)和磺胺异噁唑(80.00%)最为耐药,鸡源大肠杆菌对13种药物的耐药率高于猪源,吉林省分离菌株对10种药物的耐药率高于黑龙江省;产ESBLs菌株对13种药物的耐药率高于非产ESBLs菌株。③210株大肠杆菌多重耐药率为83.33%,猪源菌株多重耐药率(77.57%)低于鸡源(89.32%),差异显著(P=0.022),但两省之间差异不显著(P=0.360);不同动物来源、不同地区间的产ESBLs菌株多重耐药率差异均不显著(P>0.05)。④73株产ESBLs菌株主要基因型为CTX-M型(97.26%),其次为TEM型(50.68%),OXA型仅在鸡源中检出,两种来源菌株中均未检出SHV型。结果表明:产ESBLs菌株在吉林和黑龙江两省的猪鸡群中均有流行,且耐药严重,鸡源菌株比猪源耐药严重;CTX-M型和TEM型为两省猪鸡源产ESBLs大肠杆菌的主要流行基因型。本研究为指导大肠杆菌病防控的临床合理用药,有效控制产ESBLs菌株流行提供了依据。

    Abstract:

    In order to identify the distribution status and drug resistance characteristics of Escherichia coli(E. coli)strain producing extended spectrum beta-lactamases(ESBLs),and to analyze the dominant genotypes and gene subtypes,210 strains of E.coli isolated from Jilin and Heilongjiang Province were tested for ESBLs phenotypes in accordance with the standard of Clinical and Laboratory Standards Institute of America(CLSI),and its drug sensitivity and resistance genes were tested by the microdilution broth method and PCR,then data processing and statistical analysis were carried out by Chi-square test contained in SPSS software. The results showed that:i)the detection rate of ESBLs-producing E.coli strain was 34.76%,the difference of which in the two provinces was not significant(P=0.54),and that of the strains derived from chicken was significantly higher than those from pigs(P<0.01);ii)the drug resistance rate of 210 E. coli strains against tetracycline(90.95%)and sulfisoxazole(80.0%)was highest,that of chicken-derived E. coli against 13 drugs was higher than that of pig-derived E. coli,and the drug resistance rate of the strains from Jilin against 10 drugs was higher than those from Heilongjiang;and that of the ESBLs-producing strains against 13 drugs was higher than others;iii)the multiple drug resistance rate of 210 strains of E. coli was 83.33%,that of the strains derived from pigs(77.57%)was significantly lower than that of strains from chicken(89.32%),with significant difference(P=0.022),but the difference between the two provinces was not significant(P=0.360);the differences of multiple drug resistance rates of the ESBLs-producing strain in different animals and regions were both not significant(P>0.05);iv)the main genotype of 73 ESBLs-producing strains was CTX-M(97.26%)and followed by TEM(50.68%),OXA was only detected in chicken and no SHV type was detected from strains of chicken or swine origin. It was concluded that the ESBLs-producing E. coli strain was prevalent in chicken and pig populations in the two provinces,with serious drug resistance,especially for the strains derived from chicken;CTX-M and TEM were the main prevalent genotypes of E.coli derived from chicken and pigs in the two provinces. A basis was provided for guiding rational drug administration against E.coli and for effectively controlling the prevalence of ESBLs-producing strains.

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张青青,李月华,黄秀梅,赵建梅,王娟,赵格,王琳,刘娜,高玉斌,刘俊辉,张喜悦,王君玮,曲志娜.吉林和黑龙江两省猪鸡源产ESBLs大肠杆菌耐药检测与优势基因型分析[J].《中国动物检疫》编辑部,2020,37(10):42-48.

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  • 在线发布日期: 2020-09-29
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