In order to identify the distribution status and drug resistance characteristics of Escherichia coli（E. coli）strain producing extended spectrum beta-lactamases（ESBLs），and to analyze the dominant genotypes and gene subtypes，210 strains of E.coli isolated from Jilin and Heilongjiang Province were tested for ESBLs phenotypes in accordance with the standard of Clinical and Laboratory Standards Institute of America（CLSI），and its drug sensitivity and resistance genes were tested by the microdilution broth method and PCR，then data processing and statistical analysis were carried out by Chi-square test contained in SPSS software. The results showed that：i）the detection rate of ESBLs-producing E.coli strain was 34.76%，the difference of which in the two provinces was not significant（P=0.54），and that of the strains derived from chicken was significantly higher than those from pigs（P＜0.01）；ii）the drug resistance rate of 210 E. coli strains against tetracycline（90.95%）and sulfisoxazole（80.0%）was highest，that of chicken-derived E. coli against 13 drugs was higher than that of pig-derived E. coli，and the drug resistance rate of the strains from Jilin against 10 drugs was higher than those from Heilongjiang；and that of the ESBLs-producing strains against 13 drugs was higher than others；iii）the multiple drug resistance rate of 210 strains of E. coli was 83.33%，that of the strains derived from pigs（77.57%）was significantly lower than that of strains from chicken（89.32%），with significant difference（P=0.022），but the difference between the two provinces was not significant（P=0.360）；the differences of multiple drug resistance rates of the ESBLs-producing strain in different animals and regions were both not significant（P＞0.05）；iv）the main genotype of 73 ESBLs-producing strains was CTX-M（97.26%）and followed by TEM（50.68%），OXA was only detected in chicken and no SHV type was detected from strains of chicken or swine origin. It was concluded that the ESBLs-producing E. coli strain was prevalent in chicken and pig populations in the two provinces，with serious drug resistance，especially for the strains derived from chicken；CTX-M and TEM were the main prevalent genotypes of E.coli derived from chicken and pigs in the two provinces. A basis was provided for guiding rational drug administration against E.coli and for effectively controlling the prevalence of ESBLs-producing strains.