华中部分地区发病猪群血清样品PCV-2病原学检测
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国家自然科学基金资助项目(36120006);校企合作项目(34120016)


Etiological Detection of PCV-2 in Serum Samples from Infected Pigs in Some Regions of Central China
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    摘要:

    为探究华中地区临床发病猪群猪圆环病毒2型(porcine circovirus type 2,PCV-2)感染状况及特点,2018—2019年,从湖北省的武汉、随州、咸宁、荆州,湖南省的常德、衡阳、汨罗、株洲等8个地市的40个规模化猪场,采集临床发病猪群血样1 550份,利用本实验室建立的巢式PCR诊断技术进行PCV-2病原学检测,并采用SPSS 24.0卡方检验,对结果进行不同地区、不同养殖阶段、不同季节统计分析,同时追踪与其他繁殖障碍性疾病病原的混合感染情况。结果显示:湖南、湖北两省8个地区发病猪群的PCV-2平均检出率为31.68%(491/1550),所有检测地区均有不同程度的PCV-2感染,病原检出率在8.79%~49.01%之间波动,差异显著(P<0.05);不同养殖阶段发病猪群均有PCV-2阳性检出,检出率在12.17%~60.84%之间波动,差异显著(P<0.05),其中育肥猪和保育猪检出率较高,而哺乳仔猪、经产母猪和后备猪偏低;PCV-2一年四季均有检出,检出率在5.98%~49.81%之间,但有一定的季节性,夏冬季节检出率偏高,而春秋季节偏低(5.98%),差异显著(P<0.05);PCV-2和其他繁殖障碍性疾病病原的多重感染较为常见,其中以PCV-2+PRRSV双重感染和PCV-2+PRRSV+PRV三重感染为主,而四重以上感染较为少见。结果表明,湖南、湖北两省的PCV-2病原分布较为广泛,是导致该区域猪群发病的重要病因,但其流行有一定的地区、猪群和季节差异。建议加强高流行地区、高发季节、重点猪群的免疫、检测和生物安全管理,逐步控制和净化PCV-2。本研究初步了解了华中地区PCV-2的流行特点,为其流行控制和净化制定提供了参考。

    Abstract:

    In order to identify the prevalence and characteristics of porcine circovirus type 2(PCV-2)in the clinically infected pigs in Central China,1 550 blood samples collected from 40 large-scale farms in 8 municipalities including Wuhan,Suizhou,Xianning and Jingzhou of Hubei Province,and Changde,Hengyang,Miluo and Zhuzhou of Hunan Province from 2018 to 2019 were detected for PCV-2 by the nested PCR assay established in this study,then the results were analyzed as per different regions,breeding stages and seasons by SPSS 24.0 chi square test. Meanwhile,its mixed infection with other reproductive disease pathogens was tracked. The results showed that the average detection rate of PCV-2 in the above municipalities was 31.68%(491/1 550),which was distributed in different regions to different extent,the pathogen detection rate ranged 8.79% to 49.01%,with significant difference(P<0.05);positive PCV-2 was detected in infected pigs at different stages,the detection rate ranged 12.17% to 60.84%,with significant difference(P<0.05),the detection rates of fattening pigs and nursing pigs were higher than those of suckling pigs,multiparous sows and reserve pigs;PCV-2 was detected all the year round,the detection rate ranged 5.98% to 49.81%,with certain seasonality,specifically,it was higher in summer and winter,and lower in spring and autumn(5.98%),with significant difference(P<0.05);multiple infection of PCV-2 with other reproductive disease pathogens was usually found,especially the dual infection of PCV-2+PRRSV and triple infection of PCV-2+PRRSV+PRV,but quadruple infection or above was less common. It was concluded that PCV-2 was widely distributed in the two provinces,which was an important cause of pig disease in this region,but its prevalence was different in various regions,populations and reasons. So vaccination,detection and bio-security management should be strengthened in the regions at high risk,high-occurrence seasons or susceptible pig populations,for the purpose of control and purification of PCV-2. In short,the prevalence characteristics of PCV-2 in Central China were primarily identified,which provided some references for the control and purification of the disease.

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同珂,李桐,赵润泽,陈少贤,刘国平.华中部分地区发病猪群血清样品PCV-2病原学检测[J].《中国动物检疫》编辑部,2020,37(11):6-11.

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  • 在线发布日期: 2020-11-02
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