鸭圆环病毒PCR检测方法的建立与应用
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

国家重点研发计划项目(2017YFC1200503)


Development and Application of a PCR Assay for Duck Circovirus
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    鸭圆环病毒病是由鸭圆环病毒(duck circovirus,DuCV)引起的一种重要免疫抑制病,影响着我国养鸭业的发展。为建立针对DuCV的PCR快速检测方法,对GenBank中登录的DuCV全基因组序列进行遗传进化分析,通过对两种基因型的DuCV全基因组序列进行比对,在共同保守区筛选出1对引物,建立了可以同时检测DuCV两种基因型的PCR快速检测方法,并对该方法的敏感性和特异性进行评价。结果显示,该方法具有良好的敏感性,检测下限达1.0 fg;特异性良好,对其他常见鸭病毒核酸检测均为阴性;利用该方法对20份临床发病鸭样品进行检测,发现有6份呈DuCV阳性,序列分析表明均属于DuCV-1。该PCR检测方法的建立为鸭圆环病毒病的快速诊断和防控提供了重要手段。

    Abstract:

    Duck circovirus(DuCV)disease is a serious immunosuppressive disease caused by DuCV,posing a threat to the duck farming in China. In order to establish a rapid PCR assay for DuCV,the genetic evolution analysis was performed for the whole genome sequence of two DuCV genotypes that were registered in GenBank and subsequently compared with each other,then a pair of primers were screened in their common conservative area to establish a rapid PCR assay that could detect the two genotypes simultaneously. Then the sensitivity and specificity of the method were evaluated. The results showed that,by the method,the lower detection limit could reach 1.0 fg due to its good sensitivity;all nucleic acid detection for other common duck viruses were negative due to its good specificity;6 out of 20 samples of clinically infected ducks were positive for DuCV,which fell into DUCV-1 as indicated by sequence analysis. In conclusion,the method could rapidly diagnose,prevent and control DuCV as an important tool.

    参考文献
    相似文献
    引证文献
引用本文

李金平,张富友,朱峻锋,张琳,刘朔,彭程,蒋文明,刘华雷.鸭圆环病毒PCR检测方法的建立与应用[J].《中国动物检疫》编辑部,2021,38(1):106-111.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-12-30
您是第位访问者
《中国动物检疫》编辑部 ® 2021 版权所有
地址:山东省青岛市南京路369号《中国动物检疫》编辑部 邮编:266032
电话:0532-85623545 0532-85622559 0532-85642906 传真:0532-85621826 鲁ICP备08016398号-3
技术支持:北京勤云科技发展有限公司