为切实做好云南省小反刍兽疫消灭工作，保障羊产业稳定健康发展，依据《国家中长期动物疫病防治规划（2012—2020年）》和《全国小反刍兽疫消灭计划（2016—2020年）》要求，2016—2019年分别采用ELISA、RT-PCR方法，在昆明市开展了小反刍兽疫免疫抗体和病毒核酸检测。结果显示：909个集中监测养殖场点的8 972份血清样品中，检出免疫合格场点841个，平均场点免疫合格率为92.5%；检出免疫合格样品7 405份，平均个体免疫合格率为82.53%，虽然超过了农业农村部要求的不低于70%的标准，但各年度的个体合格率有下降趋势；规模场和散养户的个体免疫合格率分别为84.97%和82.07%，场点合格率分别为83.33%和93.38%，均无显著差异（P＞0.05）；不同县（市、区）的个体免疫合格率为77.36~93.17%，场点合格率为83.33%~98.55%，差异均不显著（P＞0.05）；在3 812份羊眼（鼻）棉拭子和组织样品中检出疑似核酸阳性2份，分别来自2个散养户。结果表明：昆明市小反刍兽疫整体防控效果较好，发生大面积流行的风险较低，但仍有散发疫情风险。因此，仍需继续做好小反刍兽疫的强制免疫、监测和流行病学调查工作，同时加强羊只的引种检疫和流通监管，最终到达消灭小反刍兽疫的目标。
In order to effectively eliminate peste des petits ruminants（PPR）and guarantee stable and healthy development of sheep industry in Yunnan Province，from 2016 to 2019，the PPR antibodies and viral nucleic acids were detected by ELISA and RT-PCR in accordance with the requirements specified in National Medium-long Term Plan for Animal Disease Prevention and Control（2012—2020）and National Plan for Eradication of Peste des Petits Ruminants（2016—2020）. The results showed that，a total of 8 972 serum samples were collected from 909 intensive farms/households，and 841 qualified farms/households were detected，with the average qualified rate of 92.5% at the farm/household level；7 405 qualified samples were detected，with the average qualified rate of 82.53% at the individual level，which exceeded the standard of no less than 70% required by the Ministry of Agriculture and Rural Affairs of the People's Republic of China，but the individual qualified rate trended to decrease every year；the qualified rates of scale farms and free-range households at the individual level were 84.97% and 82.07%，respectively，and the qualified rates at the farm/household level were 83.33% and 93.38%，respectively，all of which were with no obvious difference（P＞0.05）；for various counties（cities，districts），the individual qualified rates ranged from 77.36% to 93.17%，the farm/househlod qualified rates remained in the range of 83.33% to 98.55%，the difference was not significant（P＞0.05）；two suspected positive nucleic acid samples were detected from 3 812 eye（nose）cotton swabs and tissue samples，which were collected from the sheep in two different households. In conclusion，PPR was generally and effectively controlled in Kunming City，but there was still some risk of spreading，although the risk of wide prevalence was relatively low. Therefore，compulsory vaccination，monitoring and epidemiological investigation of PPR should be continued，while the quarantine and movement supervision for entry sheep should be strengthened，with a view to achieving the goal of eliminating PPR.