In order to make clear the immune dynamics and spreading risk of major animal diseases such as foot-and-mouth disease（FMD），classical swine fever（CSF），porcine reproductive and respiratory syndrome（PRRS），porcine pseudorabies（PR），brucellosis and peste des petits ruminants（PPR）in Guangxi from 2016 to 2018，the etiological and antibody surveillance was carried out for pigs，cattle and sheep from 20 fixed-point farms in Yulin City and Baise City in Guangxi in accordance with relevant requirements specified in National Animal Disease Surveillance Implementation Plan by the ELISA and real-time fluorescence quantitative PCR assay. It was shown that，by etiological surveillance，no any FMD and PPR pathogens were detected in cattle and sheep during 2016 to 2018；the detection rate of CSF pathogens was 0.38% both in 2016 and in 2017；PRRS pathogens were detected in the three years，with the detection rate ranged from 1.89% to 6.32%. It was shown that，by antibody detection，the positive rates of antibodies against FMD，CSF，PRRS and PR-gB in pigs were more than 70%，the positive rate of antibodies against FMD type A increased year by year，and that of antibodies against NSP antibody and PR-gE fluctuated；the positive rates of antibodies against FMD type O in cattle were 94.78%，18.26% and 71.11%，respectively；that of antibodies against FMD Asia-I were 76.52%，25.22% and 27.78%，respectively，and that of antibodies against NSP ranged from 22.61% to 41.74%；one Brucella-positive case in cattle was detected out only in 2016；during the three years，the positive rates of antibodies against FMD O，Asia-I type and PPR were lower than 70% with a downward trend，the positive rate of NSP antibodies fluctuated between 0.95% and 7.62%，and no antibody against Brucella in sheep was detected. In conclusion，the immune effects against FMD O type，CSF，PRRS and PR were better in pigs in Yulin and Baise cities，but those against FMD and PPR were poor in cattle and sheep，CSF，PRRS and PR were still prevalent in pigs，while FMD was in cattle and sheep，while brucellosis in cattle and sheep was effectively controlled. It was suggested that vaccination and surveillance against the above diseases should be further strengthened with a priority to prevent，control and purify the diseases with poor immune effects and high prevalence rate.