A strain of raccoon dog parvovirus（RDPV）was isolated from the feces of sick raccoon dogs in a farm in Shandong Province. In order to identify the characteristics of the isolate and its pathogenicity，virus suspension was harvested through inoculating the processed feces into F81 cells，and VP2 gene sequence analysis，physicochemical property study as well as artificial infection test were carried out. The results showed that the isolated strain belonged to canine parvovirus type 2（CPV-2），and its nucleotide homology with RDPV reference strain was up to 99.4%；it was insensitive to chloroform，but acid-resistant and heat-resistant，which conformed to the characteristics of parvovirus；the isolate was with strong pathogenicity to raccoon dogs. In conclusion，RDPV was still prevalent in Shandong Province with increasing risk of pathogenicity，and corresponding vaccines should be developed specifically to further control any virus spreading. As an outcome，epidemiological investigation on RDPV was supported with data provided in the study.