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  • 1  我国首例小反刍兽疫诊断报告
    王志亮 包静月 吴晓东 刘雨田 李林 刘佩兰 赵永刚 刘春菊 肖肖
    2007, 24(8):24-26.
    [摘要](659) [HTML](0) [PDF 0.00 Byte](86) [Cited by](38)
    摘要:
    The first outbreak of peste des petits ruminants in ovine in Tibet was confirmed by multiple laboratory tests including one step real-time RT-PCR, conventional RT-PCR, competitive ELISA and virus isolation. The presence of PPRV was detected in 11 out of 13 field samples by one step real-time RT-PCR, among which 8 were further detected by conventional RT-PCR. Sequence analysis showed that the virus fell into the lineage 4 which was epidemic in Middle East and certain western Asian countries. One strain of PPRV was isolated in Vero cell lines from field samples and confirmed by RT-PCR test.
    2  鸡腺胃型传染性支气管炎研究初报
    王玉东,张子春,王永玲,范根成,蒋贻海,刘相娥,丁江,王树双
    1997(3):6-8.
    [摘要](684) [HTML](0) [PDF 0.00 Byte](103) [Cited by](36)
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    3  我国流行的鸡致病性大肠杆菌血清型
    张春荣,苏亚拉图
    1996(1):27-27.
    [摘要](689) [HTML](0) [PDF 0.00 Byte](102) [Cited by](24)
    摘要:
    4  "猪高热病"的流行病学调查与主要病因分析
    徐辉 李晓成 陈伟杰 赵灵燕 洪建伟 周彩琴 吴发兴 顾小根 吴赟竑 倪柏锋
    2007, 24(6):19-21.
    [摘要](602) [HTML](0) [PDF 0.00 Byte](88) [Cited by](19)
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    5  抗禽流感病毒H5亚型血凝素特异性单克隆抗体的研制
    邵红霞 秦爱建 钱琨 刘岳龙 金文杰
    2002, 19(8):21-23.
    [摘要](619) [HTML](0) [PDF 0.00 Byte](92) [Cited by](18)
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    6  牙鲆鱼淋巴囊肿病初报
    曲径 江育林,沈海平,王浩,刘荭,连建华,潘雁甲,郑雪莲
    1998(2):1-3.
    [摘要](581) [HTML](0) [PDF 0.00 Byte](88) [Cited by](17)
    摘要:
    Lymphocystis disease of Paralichtys olivaceus is an infectious disease of fish caused by lymphocystis virus (LCV) of Iridoviridae.The lymphocystis virus has a wide range of hosts causing serious losses. Outbreaks of the disease occurred in six fish farms. There were distinct cysts on the surface of the infected fish body. The size of the biggest cyst was about 500um in diameter. It was the first report ot the disease in China, and the disease was suspected to be introduced by importation of fishes from other country.The integrated conrtol measures were recommended to control the epidemic and prevent it from spreading and wipe it out as soon as possible.
    7  海产品中副溶血弧菌的分离与鉴定
    马光刚 郭福生 王娟 王玉东 张衍海
    2002, 19(9):25-28.
    [摘要](775) [HTML](0) [PDF 0.00 Byte](89) [Cited by](16)
    摘要:
    Vibrio parahaemolyticus is a pathogenic halophile, present widely in bays, coastal areas, salt lakes and sea products, especially in warm regions, offten causing food poisoning of human. Tests of four species of sea products revealed high incidence of the organism, and detection rate was highest in sea shrimps, upto 90%,70% in sea fishes, 60% in molluscs, and 40% in sea crabs. Animal experiment showed that V. parahaemolyticus isolated was of ratetively high pathogenicity.
    8  伪狂犬病流行概况及猪场防制策略
    娄高明,杜伟贤
    1999(5):43-45.
    [摘要](621) [HTML](0) [PDF 0.00 Byte](86) [Cited by](15)
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    9  应用三重聚合酶链反应同时检测NDV、IBV、MG的研究
    谢芝勋,谢志勤,庞耀珊,刘加波,邓显文
    2000(11):20-22.
    [摘要](537) [HTML](0) [PDF 0.00 Byte](109) [Cited by](14)
    摘要:
    10  嗜水气单胞菌检验程序的研究
    凌红丽 陆承平 陈怀青,马向东
    1998(3):1-3.
    [摘要](616) [HTML](0) [PDF 0.00 Byte](90) [Cited by](13)
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    11  山东猪流感病毒H9N2亚型毒株的分离鉴定
    范伟兴 许传田 赵宏坤 徐龙涛 孙中诚 禚宝山
    2003, 20(8):36-37.
    [摘要](538) [HTML](0) [PDF 0.00 Byte](81) [Cited by](13)
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    12  动物源性食品中沙门氏菌的风险评估
    王军 郑增忍 王晶钰
    2007, 24(4):23-25.
    [摘要](665) [HTML](0) [PDF 0.00 Byte](91) [Cited by](13)
    摘要:
    13  β—兴奋剂正副作用及检测方法
    聂国荣
    2001, 18(6):43-44.
    [摘要](564) [HTML](0) [PDF 0.00 Byte](82) [Cited by](12)
    摘要:
    14  用PCR技术检测动物产品中沙门氏菌的研究
    沈孝民,涂书清
    1997(2):8-10.
    [摘要](535) [HTML](0) [PDF 0.00 Byte](86) [Cited by](12)
    摘要:
    15  我国牛传染性鼻气管炎血清抗体普查报告
    封启民 杨冰清 李昌琳 仰惠芬 张瑞珍 李葳 李金花
    1982(1):36-39.
    [摘要](726) [HTML](0) [PDF 0.00 Byte](93) [Cited by](12)
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    16  斑点免疫金渗滤法检测猪瘟抗体的研究
    孔繁德 黄印尧 赖清金 洪于准 陆承平
    2003, 20(11):27-29.
    [摘要](682) [HTML](0) [PDF 0.00 Byte](80) [Cited by](12)
    摘要:
    To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.
    17  猪附红细胞体病研究进展
    王君玮 王志亮 张喜悦 徐培莲
    2003, 20(7):44-46.
    [摘要](636) [HTML](0) [PDF 0.00 Byte](73) [Cited by](11)
    摘要:
    18  动物性食品中兽药残留问题及对策
    张远 刘璞
    2005, 22(6):17-19.
    [摘要](665) [HTML](0) [PDF 0.00 Byte](72) [Cited by](11)
    摘要:
    19  用PCR检测嗜水气单胞菌毒素基因
    陆承平 陈怀青
    1995(5):5-7.
    [摘要](620) [HTML](0) [PDF 0.00 Byte](83) [Cited by](10)
    摘要:
    选取气单胞菌毒素基因中保守区的同源寡核苷酸序列为引物,用PCR技术检测临床分离的嗜水气单胞菌的毒素基因。扩增产物中均含有明显的预定条带;用AluI和AvaI酶切进一步鉴定,其酶切片段的数量和大小均与设计相吻合,证实样本中带有毒素基因。细菌在鲜血平板上呈现党大的β-溶血圈,用相应的单克隆抗体检测培养上清中的毒素,亦获阳性结果,进一步证实PCR技术检测气单胞菌毒素准确可靠。
    20  用ELISA检测猪链球菌病血清抗体(初报)
    陈兴扶 徐瑞 尹燕博,陆明哲,郭福生,蒋正军,吴时友,孙淑芳,毕道荣
    1999(4):1-4.
    [摘要](568) [HTML](0) [PDF 0.00 Byte](78) [Cited by](10)
    摘要:
    An ELISA was established for thedetection of serum antibody against swine streptocosisusing 2. 5% NaCl to extract capsular polyaccharideantigen of streptococcus sui, with 2% gelatin asblocking. Solution and 4 % PEG -PBS as serum dilu-ent Test on 4 immuuized sera, 16 sera from swinestreptococcosis infected pigs, 7 sera of with pigs in-fected other diseases than swine streptococcosis and115 submitted sera for detection showed that theELISA was of high specificity and its sensilivity was25- 250 times than AGID.

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