三种伪狂犬病病毒gE抗体ELISA检测试剂盒的比较
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S852.659.1

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国家重点研发计划项目(2017YFC1200500);科技部科技基础性工作专项(2012FY111000)


Comparison on Three ELISA Test Kits for gE Antibodies of Pseudorabies Virus
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    摘要:

    为比较3种品牌(A、B、C)伪狂犬病病毒(PRV)gE抗体ELISA检测试剂盒的临床使用效果,应用中和试验来标定伪狂犬病非免疫猪场的180份临床血清样品,同时用3种试剂盒进行检测,并对试剂盒的重复性、敏感性、特异性以及与中和试验结果的符合度等指标进行测定与分析。结果显示:3种试剂盒的批内稳定性均较好,变异系数均小于10%;批间重复性差异较大,变异系数最小者为8.09%,最大者高达21.31%;3种试剂盒的诊断特性良好,敏感性为95.56%~97.78%,特异性为94.44%~100%;各试剂盒检测结果与中和试验结果均完全相符(Kappa值为0.91~0.96)。比较结果表明,3种试剂盒均适用于临床样品的PRV gE抗体检测,其中稳定性及敏感性俱佳的试剂盒C更适用于PRV的持续监测、早期发现、引种检疫及净化效果评估,而特异性与准确性最高的试剂盒B更适用于贵重病畜的诊断淘汰。因此,生产实践中应根据诊断目的合理选择最适试剂盒。

    Abstract:

    To compare the clinical using effects from three brands of commercial ELISA test kits for Pseudorabies virus(PRV)gE antibodies,180 sera samples were collected from swine farms without PRV vaccination. The sera was determined by virus neutralization test(VN)and was tested by the three ELISA kits at the same time. The characteristics of the three kits were determined and analyzed,including the repeatability,diagnostic sensitivity,specificity and coincidence with VN. The results were as follows:all the three kits had good stability in the inter-assay test with coefficient of variation(CV)below 10%,whereas they were quite different in the intra-assay test with CV,which ranged from 6.90% to 28.87%. And all of the three kits performed well in their sensitivity(from 95.56% to 97.78%)and specificity(from 94.44% to 100%). Besides,test results of the three kits were completely consistent with those of VN,with the Kappa value from 0.91 to 0.96. The results of the study indicated that all the three brands of ELISA kits were applicable for detection of PRV gE antibodies. Kit C was suitable for continuous surveillance,early detection,introduction quarantine and evaluation on eradication effect because of its best stability and sensitivity;while kit B could be used to diagnose culling expensive animals based on its best specificity and accuracy. Therefore,the optimum reagent kit should be chosen reasonably according to the purpose of diagnosis in production practice.

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董雅琴,刘爽,郑辉,张志,吴发兴,李晓成.三种伪狂犬病病毒gE抗体ELISA检测试剂盒的比较[J].《中国动物检疫》编辑部,2017,34(11):79-81.

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