斑点免疫金渗滤法检测猪瘟抗体的研究
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Studies on Dot Immunogold Filtration Assay for Detection of Antibody to Classical Swine Fever Virus
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    To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.

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孔繁德,黄印尧,赖清金,洪于准,陆承平.斑点免疫金渗滤法检测猪瘟抗体的研究[J].《中国动物检疫》编辑部,2003,20(11):27-29.

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