In this article，the application of Purification protein of tuberculin（PPD）intradermal allergy in detecting cow tuberculosis in Chenggu county was reviewed. At the same time，the problems encountered in the process were discussed and analyzed，such as the unreasonable detection time and frequency，the easily missed inspection as a result of low sampling rate，inadequate application of the detection techniques，inconsistent sensitivity of detection reagents， non-standard detection and operation and the insufficient fund for ensuing its detection and cleanup，etc. In the end，several concrete improvements were given to provide reference for the peers.