布鲁氏菌病疫苗S2株免疫成年绵羊的抗体消长规律试验
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Dynamical Changes of Antibodies after Immunization with Brucellosis Vaccine of S2 Strain in Adult Sheep
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    摘要:

    [目的]探索成年绵羊经布鲁氏菌病疫苗S2株免疫后血清抗体的消长规律。[方法]采用“灌服100亿菌”“灌服200亿菌”和“肌注50亿菌”三种不同免疫方法,对试验羊进行布鲁氏菌病S2株疫苗的免疫接种。在接种前和接种后不同时间点分别采集试验羊血清样品,用虎红凝集试验(RBT)和试管凝集试验(SAT)进行血清抗体检测。[结果]肌注组抗体上升较快,于免疫后10天达到峰值,然后快速下降,90天后下降速度减缓,但在180天时RBT和SAT阳性率仍分别为63.2%和84.2%,SAT平均凝集效价为1:178.9。2个口服组均在免疫20天后抗体达到最高,以后下降,分别于90天和120天降到最低,然后有所回升并维持在低水平波动;至180天时,口服组的RBT阳性率均下降为0,SAT阳性率分别下降为40.9%和23.1%,SAT平均凝集效价分别降至1:40.9和1:18.2。[结论] 对采用布鲁氏菌病S2株活疫苗口服免疫6个月以后的羊群,可用虎红凝集试验进行布鲁氏菌病检疫的初筛。

    Abstract:

    [Objective] In order to explore the dynamical changes of antibodies after being immunized with brucellosis vaccine of S2 strain in adult sheep.[Methods] Vaccination experiments were conducted by three different methods:perfusion of 10 billion bacteria,perfusion of 20 billion bacteria,and intramuscular injection of 5 billion bacteria. Serum samples were collected in different time-points before and after vaccination,the antibodies were detected by Rose Bengal plate agglutination test(RBT)and tube agglutination test(SAT). [Results] The antibody level after intramuscular injection rose faster than the rest,it rose up to the highest level on 10th day after immunization,then declined rapidly and descent speed became slow after 90 days. The positive rates of RBT and SAT were 63.2% and 84.2% on 180th day after immunization,and the average agglutination titer was 1:178.9. On the other side,the antibodies of oral groups reached peak on 20th day after immunization,then decreased,the minimum level appeared on the 90th and 180th day,respectively. Then they increased slightly and waved at the low level. On the 180th day,the positive rates of both two oral groups detected by RBT fell to 0%,while that by SAT dropped to 40.9% and 23.1%,respectively. [conclusion]The 6 months after oral immunization with brucellosis live vaccine of S2 strain in sheep flock,RBT could be used in preliminary screening of brucellosis inspection and quarantine.

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樊生平,齐守军,李连平,韩小军,曹春红.布鲁氏菌病疫苗S2株免疫成年绵羊的抗体消长规律试验[J].《中国动物检疫》编辑部,2017,34(2):88-90.

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  • 在线发布日期: 2017-02-23
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