[objective]To provide support for optimizing management policies by understanding current situation of the village-level animal health workers.[Methods]An investigation was carried out towards targeted groups from south，middle and north Jiangsu Province to acquire information of their identities，ages，education backgrounds，management modes and sources of subsidy. [results] In Jiangsu province，rural veterinarians often served concurrently as animal health workers，the number of which was related to the development of local animal husbandry. Most of them were at young and middle-age，with relatively low education attainments. 3 employment modes existed in these animal health workers，including signing agreements with government，being managed by clinical organizations，and commercialized operation. The financial subsidy were mostly derived from provincial and county governments，rarely from municipal finance.[conclusion] Some suggestions were put forward，such as further strengthening technical training，optimizing mechanism of subsidy and innovating the management system，so as to batter satisfy the demands of production mode transformation in animal husbandry.