羊口疮病毒QD/2015株B2L基因克隆及生物信息学分析
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S852.65

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Cloning and Bioinformatic Analysis of B2L Gene of Orf Virus QD/2015 Strain
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    摘要:

    为分析羊口疮病毒(ORFV/QD/2015株)B2L基因的分子特征,预测其编码蛋白的生物学功能,对其B2L基因进行PCR扩增、克隆及序列测定,应用生物信息学相关软件及方法,对扩增所得的基因进行序列分析,并对其编码蛋白的二级结构、细胞抗原表位、三级结构、跨膜结构域和信号肽等进行预测和分析。结果显示:ORFV/QD/2015株B2L基因序列长1 137 bp,编码379个氨基酸;该毒株与其他12株羊口疮病毒参考株的B2L基因核苷酸序列同源性为96.8%~99.7%,氨基酸序列同源性为96.8%~99.2%。系统进化分析显示,ORFV/QD/2015株与2015年分离到的ORFV/ShaanXi/2015/China株亲缘关系最近;B2L基因编码蛋白二级结构以α-螺旋区域和β-折叠区域所占比例较大,预测此蛋白可能存在7个细胞优势抗原表位,无跨膜区域,无信号肽区域;三级结构呈弯曲状螺旋结构。

    Abstract:

    In order to analyze molecular characteristics of B2L gene of Orf virus(ORFV/QD/2015) strain and to predict biological function of B2L protein. In this study,the B2L gene was amplified,cloned and sequenced. Using related bio-informatics softwares and methods,the amplified sequence was analyzed and B2L protein's secondary structure,tertiary structure,B-cell preponderant epitope,conserved domain,transmembrane domain and signal peptide were also predicted and analyzed. Results showed that the length of B2L gene was 1 137 bp,encoding 379 amino acids. The B2L gene of ORFV/QD/2015 strain shared amino acid identities of 96.8%~99.2% and nucleotide identities of 96.8%~99.7%,respectively,compared with other 12 ORFV reference strains. Phylogenetic analysis indicated a closest relationship between ORFV/QD/2015 strain and ORFV/ShaanXi/2015/China strain,the latter was isolated in 2015. Prediction of secondary structure of B2L protein indicated α-helix and β-sheet took up a large proportion.,and B2L protein possibly contained 7 potential antigen epitopes. However,no transmembrane domain or signal peptide was identified. The tertiary structure of B2L protein was curved spiral structure.

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李智,仲亮,张静,刘春羽,范俊豪,王海峰,牛云雷,张七斤.羊口疮病毒QD/2015株B2L基因克隆及生物信息学分析[J].《中国动物检疫》编辑部,2017,34(3):91-96.

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