At the end of 2011，there was an violent outbreak of Porcine pseudorabies again，which caused serious economic losses in China. In this study，1 741 swine serum samples from 56 intensive swine farms in 13 cities of Jiangsu province were collected and tested by PRV gE-ELISA and gB-ELISA antibody kits for serological epidemiological investigation. The results showed：the positive rate of wild-type PRV antibody in swine farms was 94.6%（53/56），and the rate of serum gE antibody was 43.2%，in which the northern area was obviously higher than mediate and southern areas. In addition，the positive rate of boar infected by field virus was 55%，the positive rate of gilts infected by field virus was as high as 69.4%，the positive rate of sows infected by field virus was 44%，and the positive rate of gE antibody was closely related to sow parity. The results showed that the immunization status of PRV vaccine in Jiangsu province was not optimistic，and wild virus infection was also serious，especially in the northern region of Jiangsu province. Breeding swine should be focused，and PRV vaccine immunization and antibody detection should be strengthened，so as to do the disease prevention and control better.