一株副猪嗜血杆菌的分离鉴定及其遗传进化分析
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S851.3

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甘肃省省级科技计划项目(GNSW-2007-14)


Isolation,Identification and Genetic Evolution Analysis on a Strain of Swine Haemophilus parasuis
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    摘要:

    为确认导致甘肃省平凉市一农场猪群死亡的原因,采集病死猪不同组织样品进行检测,从病死猪的脏器组织中分离到1株革兰阴性杆菌,并对其进行生化鉴定、16S rRNA鉴定及遗传进化分析,同时进行分离菌的致病性试验、耐药性分析、V因子需要试验、“卫星现象”观察。结果表明:分离菌生化特性均符合副猪嗜血杆菌;其16S rRNA基因序列与Genbank中副猪嗜血杆菌株的同源性均高达99%。因此,将该分离菌鉴定为副猪嗜血杆菌,将其命名为PL2016。动物试验及耐药性试验表明,该分离菌具有较强的致病性和多重耐药性。16S rRNA 遗传进化分析表明,该分离菌与副猪嗜血杆菌其他菌株具有很高的同源性,其核酸序列相似性高达99%以上。遗传进化分析表明,该分离菌的ompP2、sodA基因与副猪嗜血杆菌其他菌株具有很高的同源性,其核酸序列相似性高达95%以上。

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    In order to confirm the pathogenic bacteria which caused the death of swine that came from a farm of Pingliang city of Gansu province,the different tissues samples from dead swine were collected and one gram-negative,rod-shaped bacterium was isolated from viscera tissue of dead swine. Biochemical identification,16S rRNA gene sequence analysis,pathogenicity test,drug resistance analysis,V factor test and satellite phenomenon observation were carried out. The results demonstrated that all biochemical characteristics of the isolate were consistent with Haemophilus parasuis,and the 16S rRNA sequence of the isolate shared 99% homology with most Haemophilus parasuis from Genbank. Therefore,the isolate was finally identified as Haemophilus parasuis,and it was named as PL2016. Animal test and drug sensitivity test indicated that the Haemophilus parasuis strain PL2016 has stronger pathogenicity and multiple drug resistance. The genetic evolution analysis showed that the homology of 16S rRNA gene from the strain PL2016 and the other strains of Haemophilus parasuis were higher,it′s similarity of nucleotide sequence was over 99%. The genetic evolution analysis showed that the ompP2 and sodA genes from the strain PL2016 and the other strains of Haemophilus parasuis were higher homology at nucleotide level with over 95% identities.

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赵子惠,成伟伟,陈伯祥,杨明.一株副猪嗜血杆菌的分离鉴定及其遗传进化分析[J].《中国动物检疫》编辑部,2017,34(8):92-96.

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  • 在线发布日期: 2017-08-02
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