黄羽肉鸡心包积水-肝炎综合征不同组织中病毒含量的比较
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S851.3

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Comparison of Virus Content in Different Tissues of Hydropericardium Hepatitis Syndrome in Yellow Broiler Chickens
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    摘要:

    心包积水-肝炎综合征(HHS)是鸡的一种新发传染病,可导致较高的死亡率,对养鸡业构成重大威胁。本研究应用Taqman荧光PCR检测方法,对一自然病例中病死鸡不同组织样品相对病毒浓度进行了测定。结果显示不同组织平均病毒浓度从高到低依次为:肝>肺>脾>肾>肠>气管>胸腺>心>脑>心胞液>胰腺>咽/肛。不同组织间病毒浓度相差较大。其中,肝脏的病毒浓度最高,较其他组织高出约1~4个数量级,故肝脏可作为诊断、监测及疫苗制备的首选靶组织;心胞液中病毒浓度并不高,仅为肝脏的0.22%;拭子样品病毒浓度最低,提示若采用拭子样品进行检测时,漏检的机率较大,需通过增加采样数量等方法来弥补样品灵敏度不足的问题。本研究为该病的监测、诊断和研究提供了依据。

    Abstract:

    Hydropericardium hepatitis syndrome(HHS)is a novel infectious disease,causing huge economic losses in the poultry industry. In this paper,with Taqman real-time PCR method,the relative virus concentrations in different tissue samples of a natural case dead chickens were measured. The results showed that the average concentration of virus in different tissues in order from high to low were:liver> lung > spleen > kidney > intestine > trachea > thymus > heart > brain > cardiac fluid > pancreas > throat /anal swab. The virus concentration among different tissues varied greatly,in which the liver virus concentration was the highest,higher than the other tissues of about 1 to 4 in the order of 10 magnitude. So liver can be used as the preferred target tissue of diagnosis,monitoring and vaccine preparation. The virus concentration of heart cytosol was not high,only 0.22% of the livers. Swab samples of virus were the lowest. It was suggested that if the swab samples were monitored,the probability of missed detection was greater,and it was necessary to increase the number of samples and other methods to make up for the lack of sample sensitivity. This paper provides a basis for the monitoring,diagnosis and research of the disease.

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卢受昇,孙彦伟,余希尧,高慧敏,邓国东.黄羽肉鸡心包积水-肝炎综合征不同组织中病毒含量的比较[J].《中国动物检疫》编辑部,2017,34(8):104-107.

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  • 在线发布日期: 2017-08-02
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