[目的] 探讨猪瘟（CSF）、高致病性猪蓝耳病（HP-PRRS）、口蹄疫（FMD）3种疫苗不同免疫组合，对猪免疫机能及其相关细胞因子表达量的影响。[方法] 采用ELISA方法，检测猪血清中IL-1、IL-2、IFN-γ表达量的动态变化，分析免疫后3种细胞因子动态变化规律。[结果] 免疫后猪血清中的IL-1、IL-2、IFN-γ含量变化不一致。其中：IL-1表达量出现“波浪线”变化，且各试验组之间差异不显著；CSF、HP-PRRS、FMD 3种疫苗同步联合免疫组与对照组（C组）相比，二免后60 d和90 d的IL-2表达量差异显著；IFN-γ表达量呈现上升状态，3种疫苗同步联合免疫组与对照组（C组）相比，各时间点的IFN-γ表达量差异均显著（P<0.05），且维持在较高水平。[结论] CSF、HP-PRRS、FMD 3种疫苗不同免疫组合可引起猪免疫机能的变化，且对免疫机能的影响较为复杂；整个免疫过程中各细胞因子的变化不是单纯的上升和下降，而是一个动态的变化过程。
[Objective] To investigate the influence of three vaccine combinations of CSF，HP-PRRS and FMD on immune response and expression of some cytokines in swine serum. [Methods] The dynamic change of IL-1，IL-2 and IFN-γ expression in swine serum were tested by ELISA. The dynamic change rule of the three kinds of cytokines after immunization were analyzed. [Results] The change trends of IL-1，IL-2 and IFN–γ content in swine serum after immunization were not the same. A “wave”trend was appeared in the expression of IL-1，and there was no significant difference between different groups. IL-2 change trend was not consistent with different groups，combined with the group immunized by CSF，HP–PRRS and FMD vaccine，compared with the control group（group C）. The expression of IL-2 was significantly different at 60 d and 90 d after second immunization；Compared with group C，the expression of IFN–γ in the group immunized by CSF，HP–PRRS and FMD vaccine was increasing. Expressions of IFN –γ were significantly different at each time point（P <0.05），and was always at a high level. [Conclusion] Different immune combinations of CSF，HP-PRRS and FMD vaccine may cause different immune responses to swine，and the changes of cytokines throughout the immune process were not simply rising and falling，but a relatively complex dynamic change process.