[Objective] To investigate the influence of three vaccine combinations of CSF，HP-PRRS and FMD on immune response and expression of some cytokines in swine serum. [Methods] The dynamic change of IL-1，IL-2 and IFN-γ expression in swine serum were tested by ELISA. The dynamic change rule of the three kinds of cytokines after immunization were analyzed. [Results] The change trends of IL-1，IL-2 and IFN–γ content in swine serum after immunization were not the same. A “wave”trend was appeared in the expression of IL-1，and there was no significant difference between different groups. IL-2 change trend was not consistent with different groups，combined with the group immunized by CSF，HP–PRRS and FMD vaccine，compared with the control group（group C）. The expression of IL-2 was significantly different at 60 d and 90 d after second immunization；Compared with group C，the expression of IFN–γ in the group immunized by CSF，HP–PRRS and FMD vaccine was increasing. Expressions of IFN –γ were significantly different at each time point（P <0.05），and was always at a high level. [Conclusion] Different immune combinations of CSF，HP-PRRS and FMD vaccine may cause different immune responses to swine，and the changes of cytokines throughout the immune process were not simply rising and falling，but a relatively complex dynamic change process.