In order to recognize the farmers' willingness to invest in brucellosis prevention and control in the epidemic provinces，and to explore the risk factors，the multistage sampling method was used to conduct a questionnaire survey for sheep farmers in 7 provinces of mainland China，and the related data were collected，such as demographic characteristics，raising status，as well as the knowledge，attitudes，and practices in the brucellosis prevention and control. By the revealed preference method，the farmers' willingness was statistically described，and multiple linear regression model was used to analyze the influence factors. Results showed that，the average willingness to invest in brucellosis prevention and control among farmers was 0.053±0.060. The willingness was affected by factors such as age，education level，herd size，proportion of raising income，mastery of knowledge related to brucellosis and attitudes towards the culling policy，and their standardized partial regression coefficients were 0.022，0.008，0.087，0.046，0.013 and ?0.068，respectively. As a conclusion，the farmers'willingness to invest in brucellosis prevention and control was affected by many factors. Adjusting the prevention and control policies according to influence factors would help to increase the private investment willingness of farmers，and it would have positive significance for brucellosis prevention and control.