In this study，a kind of Brucella antibody detection strip was developed by the principle of colloidal gold immunochromatography. Using the national standard positive serum of Brucella，the sensitivity tests were carried out，and the lowest detection limit was determined. Meanwhile，specificity tests were also carried out using the negative samples and serum suspected of having cross reaction. Then the sensitivity and specificity of the strips at different retention time were detected，and the results showed the stable period could last for 15 months. At last，thirty clinical serum samples of cattle and sheep were selected to conduct Brucella antibody detection by this test strip and the rose-bengal plate agglutination assay simultaneously，and the compliance rate of the two reagents was 98%. As a result，the developed test trip was sensitive，specific and had long-term stability.