In this study，using the PPD method，2 106 cows in a large-scale dairy farm in Xinjiang were tested，a total of 26 cows were detected tuberculosis（TB）positive. Then whole blood derived from 20 TB-negative cows and 26 TB-positive cows were randomly collected to carry out γ-interferon ELISA test and colloidal gold test. Then the detection results were compared with PPD method. According to the comparative results，there were 16 samples detected TB-positive and 10 samples detected negative by both three methods，the detection coincidence rate was 57%. For the comparison between PPD and γ-interferon method，23 samples were detected TB-positive，with a positive coincidence rate of 88%（23/26）；11 samples were detected TB-negative，with a negative coincidence rate of 55%（11/20）. For the comparison between PPD and colloidal gold test，19 samples were detected positive，with a positive coincidence rate of 73%（19/26），19 samples were detected negative，with a negative rate of 95%（19/20）. As a conclusion，the coincidence rates among the three methods for TB detection were relatively low. Each method had its advantage and disadvantage，such as the sensitivity of γ-interferon ELISA test was highest，while colloidal gold test possessed the highest specificity. Hence，the detection method should be reasonable selected on the basis of actual requirements and needs.