禽白血病净化检测中不同样品应用的比较
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S851

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上海市科技兴农攻关项目(沪农科推字〔2018〕第4~8号)


Comparative Analysis on the Application of Different Samples in Purification and Detection of Avian Leukemia
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    摘要:

    为研究泄殖腔棉拭子、胎粪、血浆、精液、蛋清等不同检测样品在种鸡场禽白血病净化实施中的适用性和差异,将不同类型样品的禽白血病病毒p27抗原ELISA检测结果进行分组比对:一是将泄殖腔棉拭子与其他样品比对;二是以血浆病毒分离检测结果为基准,将血浆与蛋清、精液样品比对;三是将蛋清与胎粪样品比对。结果显示,泄殖腔棉拭子样品阳性率偏高,各类样品间检测结果均存在显著或极显著差异,无法相互替代。结果表明,不建议泄殖腔棉拭子样品用于禽白血病净化监测;为提高净化效率,血浆、精液、蛋清和胎粪样品应在净化检测中联合应用。

    Abstract:

    In order to study the applicability and difference of various samples including cloacal swabs,meconium,plasma,semen and egg white detected in the purification of avian leukemia in breeding chicken farms,the test results of p27 antigen of various samples by ELISA were grouped and compared as follows:cloacal swabs were compared with other samples;plasma was with egg white and semen samples based on the results of plasma virus isolation and detection;and egg white was with meconium samples. The results showed that the positive rate of cloacal swabs was relatively high,obvious or extremely obvious different results were obtained for various samples,which could not be replaced by each other. In conclusion,it was not recommended to use cloacal swabs for purification and surveillance,instead,plasma,semen,egg white and meconium samples should be jointly applied to improve efficiency of purification.

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李凯航,刘健,杨显超,赵鹏,卫龙兴,李鑫,杨德全,陶田谷晟,周锦萍.禽白血病净化检测中不同样品应用的比较[J].《中国动物检疫》编辑部,2021,38(9):77-81.

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  • 在线发布日期: 2021-09-03
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