四种牛结节性皮肤病抗体ELISA检测试剂盒的比对
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S851.3

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国家自然科学基金项目(31360601,31560707);广西农业科技项目(Z201953)


Comparison of Four ELISA Kits for Detection of Antibodies against Lumpy Skin Disease Virus
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    摘要:

    为筛选可靠的牛结节性皮肤病免疫抗体评价方法,以已知牛结节性皮肤病感染牛血清17份、羊痘疫苗免疫牛/羊血清77份和阴性牛血清20份作为样品盘,以病毒中和试验结果作为金标准,通过Kappa一致性和诊断效能评价,分别对市售的4种牛结节性皮肤病/羊痘抗体ELISA检测试剂盒进行分析比对。结果显示:4种ELISA检测试剂盒特异性为87.5%~94.34%,敏感性均不足80%,其中2种试剂盒与病毒中和试验的符合率超过80%,一致性为高度。结果表明,在临床应用中应根据检测目的选用可靠的试剂盒。本研究为科学有效防控牛结节性皮肤病提供了技术支持。

    Abstract:

    In order to select a reliable method to evaluate antibodies against lumpy skin disease virus(LSDV),17 serum samples from cattle infected with LSDV,77 serum samples from sheep and cattle vaccinated with ovinia vaccine as well as 20 negative bovine serum samples were taken as specimen discs to respectively analyze and compare four commercial ELISA kits for antibodies against LSDV/capripoxvirus through Kappa consistency test and diagnostic efficiency evaluation based on the gold criterion of results of virus neutralization test(VNT). The results showed that,for the four kits,their specificities were 87.5% to 94.34%,and sensitivities were less than 80%,and the coincidence rates of two kits with VNT were above 80%,and the consistencies were identified as“high”. In conclusion,a reliable kit should be selected according to specific purpose of detection in practice. A technical support was provided in the paper for scientific and effective prevention and control of LSDV.

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闭璟珊,赵钟毅,吕荞,韦正吉,邹联斌,苏姣秀,尹德炜,郑敏.四种牛结节性皮肤病抗体ELISA检测试剂盒的比对[J].《中国动物检疫》编辑部,2021,38(9):82-86.

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  • 在线发布日期: 2021-09-03
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