鸭源鸡杆菌平板凝集检测方法的建立与应用
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S852.61+4

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国家重点研发计划滚动支持项目(2020YFF0426460)


Establishment and Application of a Plate Agglutination Assay for Gallibacterium anatis
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    摘要:

    为建立一种方便、快捷的鸭源鸡杆菌血清抗体诊断方法,通过用鸭源鸡杆菌YT-1株制备抗原,然后免疫健康家兔制备阴阳性血清,建立了鸭源鸡杆菌平板凝集检测方法,并进行特异性试验,确定抗原最佳工作浓度,同时用该方法进行临床样品检测。结果显示:鸭源鸡杆菌染色抗原与鸡源阳性血清产生明显凝集,而与鸡源阴性血清以及鸡大肠杆菌、鸡沙门氏菌、副鸡嗜血杆菌、禽流感病毒、鸡新城疫病毒、鸡传染性支气管炎病毒和禽腺病毒等病原的阳性血清均不凝集;当抗原浓度为5×109 CFU/mL时,抗原与血清凝集最佳。结果表明,建立的鸭源鸡杆菌血清抗体平板凝集试验方法具有良好的特异性,可用于开产前后鸡群鸭源鸡杆菌的检测评估及流行病学调查。

    Abstract:

    In order to establish a convenient and rapid method for detecting serum antibodies against Gallibacterium anatis(G. anatis),antigens were prepared by using YT-1 strain of G. anatis,followed by negative and positive serum by immunized healthy rabbits,then a plate agglutination assay was established,meanwhile,its specificity was evaluated and the optimal concentration of antigens were determined,and clinical samples were tested by the established method. The results showed that the staining antigen of G. anatis agglutinated significantly with chicken positive serum,but failed with chicken negative serum and positive serum of pathogens including avian Escherichia coli,Salmonella,Haemophilus paragallinarum,avian influenza virus,newcastle disease virus,avian infectious bronchitis virus and avian adenovirus;the agglutination reached up to the best when the antigens concentration was 5×109 CFU/mL. It was concluded that the established method was with good specificity and could be used for detection,evaluation and epidemiological investigation of G. anatis in chicken flocks prior to or after egg production.

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张玉杰,刘红祥,刘东,张翔,杜元钊.鸭源鸡杆菌平板凝集检测方法的建立与应用[J].《中国动物检疫》编辑部,2021,38(10):115-119.

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  • 在线发布日期: 2021-10-08
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