狮源猫细小病毒分离鉴定及其VP2基因序列分析
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S852.65

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广东省科技攻关应急专项(2020B111320002)


Isolation and Identification of Lion-derived Feline Parvovirus and Analysis on VP2 Gene Sequence
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    摘要:

    为对疑似感染猫细小病毒的非洲狮进行确诊,了解猫细小病毒的流行特点,通过猫肾细胞(F81细胞)对非洲狮粪便样品进行病毒分离并通过PCR鉴定,对检测到的细小病毒进行VP2基因扩增和分析。结果显示:成功从非洲狮粪便中分离出1株细小病毒,将其命名为FPV CHN-HN-1。该病毒接种到F81细胞上,能使细胞出现拉丝、脱落现象。该分离株与参考的26株细小病毒分离株相似性为97.8%~100%,其中与分离株JN-FPV-96(MZ836373.1)、TZ-FPV-112(MZ836368.1)、FPV-SH2003(MW811187.1)和BJ051(MT270580.1)相似性高达100%;与其他7株猫源细小病毒同属一个分支,与JN-FPV-96、TZ-FPV-112、FPV-SH2003、BJ051遗传距离最近,与其他参考毒株遗传距离较远。本研究为细小病毒病的诊断和治疗提供了依据,也为后期疫苗的研制打下了基础。

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    In order to confirm a suspected case of African lions infected with feline parvovirus,and to identify the prevalent characteristics of feline parvovirus,the virus was isolated from fecal samples of African lions through kidney cells(F81 cells)and identified by PCR,followed by VP2 gene amplification and analysis. The results showed that one strain of parvovirus named as FPV CHN-HN-1 was successfully isolated from the samples. The isolated virus was inoculated to F81 cells,and the cells could be wiredrawn and fall off. The isolate shared the similarity of 97.8% to 100% with 26 reference strains,and up to 100% with the isolates of JN-FPV-96(MZ836373.1),TZ-FPV-112(MZ836368.1),FPV-SH2003(MW811187.1)and BJ051(MT270580.1);the isolate belonged to the same branch as other 7 strains of feline parvovirus,its genetic distance from JN-FPV-96,TZ-FPV-112,FPV-SH2003 and BJ051 was minimum,and maximum from other reference strains. In the paper,a basis was provided for diagnosis and control of feline parvovirus,and a foundation was laid for future development of related vaccines.

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吕梦娜,韦柳明,单芬,彭仕明,陈武,翟俊琼.狮源猫细小病毒分离鉴定及其VP2基因序列分析[J].《中国动物检疫》编辑部,2021,38(11):118-123.

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