为了解鸡喉黏膜不同时期的免疫状态，采集不同日龄海兰白鸡的喉组织，利用免疫组织化学方法研究CD3+ T淋巴细胞和Bu-1+ B淋巴细胞的出现、定位分布及数量变化过程。结果显示：4日龄时，CD3+ T淋巴细胞和Bu-1+ B淋巴细胞都首次出现在喉黏膜中，而且都主要分布在喉腔底壁的正中黏膜嵴中；7日龄时喉黏膜中CD3+ T淋巴细胞和Bu-1+ B淋巴细胞急剧增多，并形成淋巴聚集物，CD3+细胞占据淋巴聚集物的中下部区域，而Bu-1+细胞主要位于淋巴聚集物外周；14日龄时Bu-1+细胞虽仍主要分布于CD3+细胞的外周，但分界不如7日龄时明显，有部分Bu-1+细胞穿插分布于CD3+细胞之间；21日龄和35日龄时，喉黏膜固有层中淋巴细胞更为集中；56日龄时，黏膜固有层底端出现主要由Bu-1+ B淋巴细胞构成的生发中心，而CD3+ T淋巴细胞则分布在生发中心周围的滤泡间区域。在数量变化上，随着日龄增长，CD3+ T淋巴细胞和Bu-1+ B淋巴细胞数量均逐渐增加，且14日龄之前CD3+细胞数量多于Bu-1+细胞，而此后各日龄Bu-1+细胞的数量均显著多于CD3+细胞（P＜0.05）。结果表明，鸡喉黏膜中T、B淋巴细胞的分布和数量均呈现日龄相关性变化，并且其变化可以反映出14日龄之前喉黏膜以细胞免疫为主，而之后则倾向于体液免疫。本试验为进一步研究家禽喉黏膜的免疫机制奠定了基础。
In order to identify the immune status of laryngeal mucosa of chickens at different stages，laryngeal tissues were collected from Hailan white chickens at different days old to study the occurrence，location，distribution and number changes of CD3+ T and Bu-1+ B lymphocytes by an immunohistochemical method. The results showed that the two kinds of lymphocytes appeared in the laryngeal mucosa for the first time when the chickens were at 4 day-old，and they were mainly distributed in the median mucosal crest at the bottom wall of laryngeal cavity；and sharply increased at 7 day-old to form lymphoid aggregates，CD3+ cells were located in the middle and lower lymphoid aggregates，while Bu-1+ cells were mainly around their periphery；Bu-1+ cells were still mainly distributed around the periphery of CD3+ cells at 14 day-old，but their boundary was not as obvious as that at 7day-old，and some Bu-1+ cells were interspersed and distributed among CD3+ cells；lymphocytes were more concentrated in the lamina propria of laryngeal mucosa at 21 and 35 day-old；at 56 day-old，a germinal center mainly composed of Bu-1+ B lymphocytes was found at the bottom of the lamina propria of mucosa，while CD3+ T lymphocytes were distributed in the interfollicular area around the germinal center. The number of CD3+ T and Bu-1+ B lymphocytes increased gradually with the increase of day-old，and the number of CD3+ cells was more than that of Bu-1+ cells prior to 14 day-old，however，subsequently，the number of Bu-1+ cells was significantly more than that of CD3+ cells（P＜0.05）. In conclusion，the distribution and number of T and B lymphocytes in laryngeal mucosa correlatively changed with day old. Based on the changes，it could be found that the laryngeal mucosa was dominated by cellular immunity prior to 14 day-old，and then humoral immunity was preferred. A foundation was thereby provided for further study on the immune mechanism of poultry laryngeal mucosa.