5株湖南猪圆环病毒1型全基因组测序与遗传进化分析
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S852.65

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湖南省生猪产业体系岗位专家基金项目


Whole Genome Sequencing of Five Strains of PCV1 in Hunan Province and Analysis on Their Genetic Evolution
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    摘要:

    为了解湖南猪群猪圆环病毒1型(PCV1)的遗传变异情况,以PCV1阳性DNA为模板,使用PCR方法分两段扩增PCV1全基因序列并进行拼接。结果共获得5个PCV1全基因组,其中3株全基因大小为1 759 bp,另外2株分别为1 758 bp和1 760 bp。5株全基因组同源性为98.4%~99.7%,与国内外的PCV1全基因组同源性为98.1%~99.7%;ORF2序列同源性为97.6%~99.6%,与国内外的PCV1 ORF2同源性为96.1%~99.9%。系统发育树显示,5株PCV1均属于同一分支,并显示出一定的地理差异,但差异较小。结果表明,湖南省流行的PCV1毒株基因较为稳定,分离株间差异较小。本研究为湖南省猪圆环病毒病防控及相关研究奠定了基础。

    Abstract:

    In order to identify the genetic variation of porcine circovirus type 1(PCV1)in pigs in Hunan Province,taking PCV1 positive DNA as a template,two sections of PCV1 gene sequence were amplified by PCR and then spliced together to form the whole genome. Five strains of whole genomes were obtained,in which,three were 1 759 bp,and two were 1 758 bp and 1 760 bp,respectively. The homology of the whole genome was 98.4% to 99.7%,which shared the homology of 98.1% to 99.7% with other whole genomes of PCV1;the homology of ORF2 sequence was 97.6% to 99.6%,which shared the homology of 96.1% to 99.9% with other PCV1 ORF2. As indicated by the phylogenetic tree,all the five strains belonged to the same branch with slightly geographical difference. In conclusion,the strains were steadily prevalent in Hunan Province with little difference. A foundation was thereby provided for future prevention and control of PCV in Hunan and for relevant studies.

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何世成,王紫微,王成,胡巧云,唐小明,王卫国,谢怡灵,王昌建.5株湖南猪圆环病毒1型全基因组测序与遗传进化分析[J].《中国动物检疫》编辑部,2021,38(12):26-32.

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  • 在线发布日期: 2021-12-03
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