云南省野猪源猪瘟病毒E2及5'NTR基因序列测定分析
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S852.65

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云南省重大科技专项计划项目(202102AE090039);云南省重点研发计划项目(20180BB004)


Sequencing and Analysis of E2 and 5'NTR Genes of CSFV Derived from Wild Boars in Yunnan Province
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    摘要:

    猪瘟(CSF)是由猪瘟病毒(CSFV)感染猪引起的一种急性、热性、接触性传染病,严重危害我国养猪业健康稳定发展。本研究采用RT-PCR方法,对从云南省宁洱县2头病死野猪体内分离到的2株CSFV毒株进行了E2及5'NTR基因扩增、克隆及序列测定;采用DNAMAN、MEGA6等分子生物学软件,对测得的序列与国内外参考毒株进行了同源性分析及系统发育分析。结果显示:2株野猪源CSFV株E2、5'NTR基因同源性分别为87.2%、100%,与国内外参考毒株同源性分别为81.0%~97.6%、93.6%~98.5%,与我国强毒株Shimen株的同源性分别为81.0%~81.2%、95.6%;2株野猪源CSFV毒株E2基因属于基因2.1亚型,5'NTR基因属于基因2.3亚型。氨基酸序列分析显示:其中一株分离毒株的E2基因有6个氨基酸具有2.1d分子变异特征,另一株兼有2.1d和2.1b亚型分支特征,可能是2.1b和2.1d亚型的过渡毒株。结果表明,云南省野猪源CSFV虽存在一定的遗传衍化,但总体比较稳定。本研究为云南省CSF防控提供了分子流行病学依据,为进一步做好分子变异等研究奠定了基础。

    Abstract:

    Classical swine fever(CSF)is an acute,febrile and contact infectious disease caused by classical swine fever virus(CSFV),which has seriously endangered the healthy and stable development of pig industry in China. In this study,E2 and 5'NTR genes of two CSFV strains isolated from two dead wild boars in Ning'er County of Yunnan Province were amplified,cloned and sequenced by RT-PCR;the homology and phylogeny of the obtained sequences and the reference strains were analyzed and compared by DNAMAN,MEGA6.0 and other molecular biology software. The results showed that the homology of E2 and 5'NTR gene of the two strains were 87.2% and 100%,respectively,which were 81.0% to 97.6% and 93.6% to 98.5% respectively with the reference strains,81.0% to 81.2% and 95.6% respectively with Shimen virulent strains in China;for the two strains,E2 gene and 5'NTR gene belonged to subtype 2.1 and subtype 2.3,respectively. As indicated by amino acid sequence analysis,6 amino acids with molecular variation characteristics of 2.1d subtype were observed in one strain,characteristics of both 2.1d and 2.1b subtypes were in another strain,which might be a transitional strain of the two subtypes. In conclusion,the CSFV prevalent in wild boars in Yunnan Province was generally steady although a certain genetic evolution was detected. A molecular epidemiological basis was provided for prevention and control of CSF in Yunnan Province,and a foundation was also laid for further studies on molecular variation.

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赵焕云,张海楠,鲁富有,解明华,曾邦权,陈云明,吕嵘,李锡慧,张文东.云南省野猪源猪瘟病毒E2及5'NTR基因序列测定分析[J].《中国动物检疫》编辑部,2021,38(12):33-37.

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