In order to investigate the infection of Toxoplasma gondii in cattle in Chenzhou City of Hunan Province，and to find out its possible risk factors，710 serum samples were collected from 9 cattle farms in the region to detect antibody against Toxoplasma gondii by indirect hemagglutination test（IHA），to calculate the positive rate by the statistical method，and to conduct Chi-square test for different risk factors using SPSS V23.0 software. The results showed that 64 positive samples were detected，with positive rate of 9.0%；all detected farms were infected，especially the farms in Zixing City（14.5%）；the positive rates in springs（12.9%）and summers（13.7%）were higher than those in falls（5.1%）and winters（4.1%），with obvious difference（P＜0.05）；the positive rates were 8.9% and 9.1% respectively in intensive farms and free-range households，with slightly difference（P＞0.05）；higher in the farms with poor health conditions（12.9%）compared to those with good conditions（7.4%），with obvious difference（P＜0.05）；slightly higher in female cattle（9.1%）compared to the male ones（8.6%），with little difference（P＞0.05）；highest in the cattle above 3-year old（14.8%），and 7.8% to 9.7% in those less than 3-year old，with slightly difference（P＞0.05），although all cattle could be infected；and was 12.8% in the farms where cats were available，and 2.9% in those without any cat，with obvious difference（P＜0.05）. The risk of Toxoplasma gondii infection was about 8.4 times in the farms in north Chenzhou City higher than that in the west，8.6 times in the south than that in the west，4.6 times in the middle than that in the west，4.8 times in the farms with cats than those without cats，3.7 times in autumns than in winters，and 1.9 times in the farms with poor conditions than in those with good ones. In conclusion，Toxoplasma gondii was widely distributed in the farms in Chenzhou City，and its main infection risk factors included geographical location of the farms，the presence of cats，seasons and health conditions in the farms，corresponding measures should be accordingly developed based on the above risk factors to prevent and control toxoplasmosis，and to ensure human safety.