宁夏中南部地区牛支原体病原学检测及分离鉴定
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S852.62

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宁夏重点研发计划现代农业科技创新示范区专项(2019BBF02005);宁夏动物疫病净化科技创新团队项目;宁夏回族自治区青年拔尖人才培养工程项目


Etiological Detection,Isolation and Identification of Mycoplasma bovis in Central and Southern Ningxia
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    摘要:

    为了解宁夏中南部地区牛支原体感染及遗传变异情况,采用PCR方法,对来自宁夏中南部9个县区规模场、屠宰场和交易市场的495份牛鼻拭子样本进行PCR检测及菌株分离鉴定。结果显示:495份样本中,经PCR检出阳性59份,总体阳性率为11.92%,9个县区的阳性率为1.82%~21.82%,规模场、屠宰场、交易市场阳性率分别为11.11%、13.33%、12.59%;共分离出16株疑似牛支原体菌株,经oppF基因特异性引物扩增、测序比对及建立分离株系统发育树,结果有4株被确定为牛支原体;进一步用分离株16S rRNA序列与支原体代表株构建系统发育树,结果发现分离菌株与国际标准株PG45、宁夏菌株Ningxia-1亲缘关系最近。药敏试验发现,分离菌株对苯唑西林、链霉素、环丙沙星、多黏菌素B、阿莫西林、哌拉西林、头孢唑林、氨苄西林不敏感。结果表明,宁夏中南部地区牛支原体流行较为普遍,流行菌株未发生较大的基因变异,但对部分抗生素产生了耐药。建议宁夏中南部地区加强对牛支原体病的防控,临床防治时要结合药敏试验,选用敏感药物,适当联合用药和穿插用药,避免产生耐药性。本研究为宁夏中南部地区牛支原体病防控和临床用药提供了参考。

    Abstract:

    In order to investigate the infection and genetic variation of Mycoplasma bovis in central and southern Ningxia,495 bovine nose swabs collected from intensive farms,slaughterhouses and trading markets in nine counties/districts in the region were detected by PCR and identified through strain isolation. The results showed that 59 positive samples were detected,with an overall positive rate of 11.92%,the positive rate of all the counties/districts was 1.82% to 21.82%,specifically,11.11%,13.33% and 12.59% for intensive farms,slaughterhouses and trading markets,respectively;16 suspected strains of Mycoplasma bovis were isolated,then amplified by oppF gene-specific primers,sequenced and analysed to establish a phylogenetic tree,4 strains were identified as Mycoplasma bovis;the phylogenetic tree was further established by 16S rRNA sequence of the isolated strain and the representative strain of Mycoplasma bovis,and it was observed that the strain was most closely related to the international standard strain PG45 and Ningxia strain(Ningxia-1). After drug sensitivity test,it was found that the isolated strain was not sensitive to oxacillin,streptomycin,ciprofloxacin,polymyxin B,amoxicillin,piperacillin,cefazolin and ampicillin. In conclusion,Mycoplasma bovis was widely distributed in central and southern Ningxia,and no major genetic variation was observed in the prevalent strains that were resistant to some antibiotics. It was thus recommended to strengthen the prevention and control for bovine mycoplasma disease,and select sensitive drugs with joint and interspersed administration in combination with drug sensitivity test during clinical prevention to avoid any drug resistance. A reference was therefore provided for future prevention and control for bovine mycoplasma disease and clinical administration in the region.

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马林,周海宁,杨佳冰,马龙,闵泽,魏凡华,李知新.宁夏中南部地区牛支原体病原学检测及分离鉴定[J].《中国动物检疫》编辑部,2022,39(5):36-41.

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  • 在线发布日期: 2022-05-07
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