In order to investigate the prevalence and molecular genetic variation of rabbit hemorrhagic disease virus type 2（RHDV2）in Sichuan Province，a total of 7 525 samples were collected across the province for RHDV2 detection by commercial RT-PCR kits，including 117 rabbit liver tissues and 243 environmental swabs from the infected farms，as well as 3 340 blood and 3 825 environmental swabs from the monitored farms. 12 positive liver tissues were screened for RT-PCR amplification，sequencing and genetic evolution analysis of RHDV2 VP60 gene. The results showed that no obvious seasonality was observed in the prevalence of RHDV2，and all breeds of rabbits could be infected，the mortality rates of neonatal rabbits，young rabbits and pregnant or lactating rabbits were 25% to 60%，18% to 45% and 9% to 30%，respectively. The positive rate was 88.89%（104/117）in liver tissues and 46.09%（112/243）in environmental swabs from infected farms，25.99%（868/3 340）in whole blood and 16.29%（623/3 825）in environmental swabs from monitored farms. For 12 positive samples，the length of RHDV2 VP60 gene sequence was 1 740 bp，and the nucleotide sequence homology was 98.4% to 100%，which formed a single cluster in phylogenetic tree；their nucleotide homology with the reference RHDV2 VP60 gene sequence was 93.7% to 100%，and the encoded amino acid homology was 95.7% to 99.8%. In conclusion，RHDV2 had started to spread across the province，for which quarantine and feeding management should be strengthened；and its VP60 gene sequence varied to a certain extent，but its genetic evolution was relatively stable. The molecular epidemiological information related to RHDV2 was supplemented by the study，which also provided a reference for the development of relevant prevention and control measures for the disease.