In order to identify the carrying status，drug resistance and pathogenicity of avian Campylobacter jejuni（C. jejuni）in different processes，600 samples were collected from broiler breeding and slaughtering processes，and 241 strains of C. jejuni were isolated to detect their drug resistance and virulence genes by micro broth dilution and PCR. The results showed that the isolates were strongly resistant to ciprofloxacin，naphthalidonic acid and tetracycline，with the drug resistance rates of 97.75%，96.78% and 95.18%，respectively；the resistance in breeding was more serious than that in slaughtering，with the resistance rate up to 100% to ciprofloxacin and tetracycline，98.67% to naphthyridine acid，and to azithromycin，erythromycin and talicamycin，35.00% higher than those in slaughtering；except VirBII gene that was not detected and neuB gene with lower carrying rate，the carrying rates of other 14 virulence genes were high，and above 90% in 11 genes. The isolates were vaccinated into mice for pathogenicity test. It was found that the isolates could produce certain pathogenicity to mice. In conclusion，C. jejuni was seriously resistant to drugs with certain pathogenicity，which should be further studied and controlled.