In order to implement the Bio-safety Law of the People's Republic of China and the spirit of relevant documents about strengthening the management of animal pathogenic microbiology laboratories issued by the Ministry of Agriculture and Rural Affairs，a thorough investigation and special inspection on bio-safety of the laboratories were conducted in Anhui Province. It was investigated that，for 103 laboratories，a bio-safety management committee was organized to clarify the respective responsibilities of bio-safety responsible persons，supervisors and administrators，to establish or improve bio-safety management system，emergency plan and response mechanism，to conduct risk assessment regularly and to apply correction measures in time if necessary. Some problems were also observed，such as insufficient bio-safety facilities and equipment，imperfect bio-safety management system，inadequate bio-safety management measures，etc.，against which，some suggestions were put forward，including strengthening the publicity and implementation of the Bio-safety Law of the People's Republic of China and continuing to promote filing of animal pathogenic microorganism laboratories，upgrading bio-safety facilities and equipment，improving bio-safety management systems，enhancing hardware and software construction，increasing trainings on bio-safety management and strengthening supervision and guidance.