流浪犬狂犬病口服疫苗免疫效果评估
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S855.3

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上海市科技兴农重点攻关项目(2020-02-08-00-03-F01460);2018上海农业领军人才项目


Evaluation on the Immune Effect of Oral Vaccine against Rabies in Stray Dogs
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    摘要:

    为了解狂犬病病毒CAV2-ΔE3-CGS口服疫苗在流浪犬体内产生的抗体水平,探索抗体产生规律,选取上海市收容的12只流浪犬开展口服疫苗免疫试验,按照免疫程序采用间接ELISA方法测定狂犬病病毒免疫抗体。结果显示:CAV2-ΔE3-CGS口服疫苗一免和二免后平均抗体水平分别达到0.23和0.81 IU/mL;口服疫苗平均抗体水平总体呈上升趋势,一免后不能达到保护水平,二免后抗体水平迅速升高,60%犬只狂犬病抗体达到保护水平(0.50 IU/mL)。研究表明,CAV2-ΔE3-CGS口服疫苗可以使流浪犬产生狂犬病病毒抗体,二免后60%犬只抗体水平能达到国际标准要求,产生完全保护作用。本试验探究了CAV2-ΔE3-CGS口服疫苗的免疫效果,为今后流浪犬狂犬病免疫方案和免疫程序的制定提供了数据支持。

    Abstract:

    In order to investigate the antibody level produced in stray dogs by rabies virus CAV2-ΔE3-CGS oral vaccine,and to explore the rule for producing antibody,12 stray dogs in Shanghai were selected for oral vaccine immunization test and to determine the immune antibody by indirect ELISA according to the immunization procedure. The result showed that the qualified rate of antibody produced by CAV2-ΔE3-CGS oral vaccine was 60%,the average antibody levels after primary and secondary immunization reached 0.23 and 0.81 IU/ml,respectively;the average antibody level of oral vaccine tended to increase in general,specifically,the level of protection could not be reached after primary immunization,but reached rapidly after secondary one,and were up to the required level(0.50 IU/mL)in 60% dogs. In conclusion,the antibody against rabies virus could be produced in stray dogs by CAV2-ΔE3-CGS oral vaccine,and the antibody level of 60% dogs could meet the international standard after secondary immunization,and thus provided protection. The immunity of CAV2-ΔE3-CGS oral vaccine was studied in the paper,which provided data support for future development of immunization program and procedure against rabies in stray dogs.

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白艺兰,杨显超,刘健,夏炉明,陈伟锋,龚国华,朱晓英,陶田谷晟,常晓静,桂亚萍,李增强,王建,赵洪进.流浪犬狂犬病口服疫苗免疫效果评估[J].《中国动物检疫》编辑部,2022,39(5):116-120.

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  • 在线发布日期: 2022-05-07
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