In order to investigate the infection of enzootic nasal tumor virus（ENTV）in goats in Yunnan Province，goat blood was collected in 2017 to isolate plasma in Jiangcheng County，the southern border area of Yunnan Province，followed by amplification by random PCR and clone，then two sequences with high homology with ENTV-2 were obtained from 20 plasma samples；a set of semi-nested primers were designed based on the sequence of ENTV-2 CHN1（KU258870）to detect the nucleic acid of 20 plasma samples，in which four were positive. It was found that，by sequence analysis，the four sequences were located in the same evolutionary clade as ENTV-2 prevalent in Sichuan and Fujian provinces，with nucleotide homology of 96.3% to 99.5% and amino acid homology of 95.3% to 99.0%，which indicated that ENTV-2 prevalent in goats in the county might be related to those in Sichuan and Fujian provinces；by site analysis，a unique“G→E”amino acid mutation was observed in the four strains when they were located at the positions of 186 and 204 of env protein，and“T→P”in one strain at the position of 178. In conclusion，goats in the county were infected with ENTV-2 that showed some unique molecular characteristics. Therefore，ENTV should be strictly monitored to identify its prevalence and rule of evolution in goats in the province，and then to provide a basis for further efforts against the disease.