北京市密云区非洲猪瘟病原监测的做法及意义
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S851.3

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Practice and Significance of Nucleic Acid Detection of African Swine Fever Virus in Miyun District,Beijing City
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    摘要:

    为防控非洲猪瘟疫情的传入和发生,北京市密云区针对本辖区的生猪养殖和地理特点,在消毒、封闭管理、严格运输检疫等常规措施下,对养殖、运输、屠宰、生产加工流通和无害化处理等环节进行定期、定点采样,应用实时荧光定量PCR方法进行非洲猪瘟病毒核酸检测。2019—2021年对所有生猪养殖场每栋必检,共检测样品75 600份,出栏生猪批批检测,共检测样品4 245份;病死猪存放点每7 d检测1次,共检测样品2 611份;调入白条猪肉批批检,共检测样品14 000份;备案运输生猪车辆定期检测,共检测样品229份;屠宰厂运输车辆定期检测,共检测样品37份;共检测屠宰厂环境样品53份,病死猪存放点冷库、冰柜样品396份。以上样品的非洲猪瘟病毒核酸检测均为阴性,说明在常规防控措施下,重点开展非洲猪瘟病毒核酸检测的做法取得了极好的非洲猪瘟防控效果。非洲猪瘟病原监测可促使养殖到屠宰加工各环节的自我防控意识提高,促进运输车辆出入时严格消毒,调入猪肉产品时严格检疫等措施的落实。密云区非洲猪瘟病原监测的做法为其他地区的非洲猪瘟防控提供了借鉴。

    Abstract:

    In order to prevent and control the introduction or occurrence of African swine fever(ASF),based on the characteristics of local pig breeding and geographical characteristics in Miyun District of Beijing City,the links of breeding,transportation,slaughter,production,processing,circulation and harmless treatment were regularly sampled at fixed points with disinfection,closed-off management,strict quarantine for transportation and other conventional measures,followed by nucleic acid detection of ASF virus(ASFV)by real-time fluorescence quantitative PCR. From 2019 to 2021,all farms were detected,involving 75 600 samples,and all live pigs for slaughter were detected in batches,involving 4 245 samples;all storage points for dead pigs were detected every 7 days,involving 2 611 samples;all introduced pig carcasses were detected lot by lot,involving 14 000 samples;registered vehicles transporting live pigs and those in slaughterhouses were regularly detected,involving 229 and 37 samples,respectively;53 environmental samples from slaughterhouses and 396 from refrigerators and freezers in the storage points for dead pigs were detected. All the above samples were negative,which indicated that excellent effects against ASF were achieved with a priority of nucleic acid detection of ASFV under conventional measures. Due to the detection,the awareness of self prevention and control in all links from breeding to slaughtering or processing could be improved,vehicles could be strictly disinfected when they enter or exit,and pork products could be strictly inspected when they were introduced. A reference was thus provided for the prevention and control of ASF in other regions.

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王雅先,温富勇,于桂芳,张美晶,李艳,胡琦,李春刚,卢小东.北京市密云区非洲猪瘟病原监测的做法及意义[J].《中国动物检疫》编辑部,2022,39(6):81-85.

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